J Biomed Res.
2013 Jun;14(2):99-104.
Differentially expressed proteins in the liver of Gulo-/- mice following treatments with Helicobacter pylori and diethylnitrosamine
- Affiliations
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- 1Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea. gonskim@gnu.ac.kr
- 2Department of Biological Science, Center for Colon Cancer Research, University of South Carolina, Columbia, SC, 29208, USA.
- 3Korea National Animal Research Resource Center, Korea National Animal Bio-resource Bank, Gyeongsang National University, Jinju 660-701, Korea.
- 4Department of Nursing Science, International University of Korea, Jinju 660-759, Korea.
- 5Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-701, Korea.
- 6Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Korea.
- 7Department of Microbiology, Gyeongsang National University School of Medicine, Jinju 660-701, Korea.
Abstract
- Vitamin C (ascorbic acid) is an essential nutrient of most living tissues. We established a strain of Gulo-/- mice with known deficiency, in which vitamin C intake can be controlled by diet, like humans, and investigated the differentially expressed proteins following treatments with Helicobacter pylori and diethylnitrosamine (DENA) in the liver of Gulo-/- mice using a proteomic approach. Expression of p53, 14-3-3epsilon and 14-3-3delta in Gulo-/- mice liver tissue was analyzed by immunohistochemistry. 2-DE maps constructed from Gulo-/- mice liver and differentially expressed proteins in liver tissue were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS). In Gulo-/- mice after H. Pylori infection, followed by treatment with DENA, no differences in p53, 14-3-3epsilon and 14-3-3delta were observed by immunohistochemistry. Proteome analyses using MALDI-TOF/MS resulted in successful identification of 12 proteins (nine proteins were up-regulated and three were down-regulated). Specifically, peroxiredoxin-6 and Alpha-1-antitrypsin 1-4 were up-regulated in liver after H. Pylori infection followed by treatment with DENA. These results indicated that oral supplementation with vitamin C led to rescue of Gulo-/- mice from vitamin deficiency, and protected the liver from H.pylori infection and/or DENA effect, and vitamin C also protected the liver against oxidative stress.
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