Mycobiology.  2008 Sep;36(3):183-189. 10.4489/MYCO.2008.36.3.183.

Production of Aerial Conidia of Lecanicillium lecanii 41185 by Solid-State Fermentation for Use as a Mycoinsecticide

• Vu VH
• Hong SI
• Kim K

• KMID: 1487341
• DOI: http://doi.org/10.4489/MYCO.2008.36.3.183

Abstract

The production of aerial conidia of Lecanicillium lecanii 41185, a highly virulent fungus, by solid-state fermentation was studied for use as a biocontrol agent against aphids. Among several agro-industrial solid media, steamed polished rice was found to produce the highest amount of aerial conidia. The optimal conditions for aerial conidia production were determined to be a 28.5% moisture content in the rice, 25degrees C culture temperature, rice pH of 6.0, 75% ambient relative humidity, 4-dold seeding culture, 0.6% KNO3, and 12 d of culture time. The conidia yield increased from 5.7 x 10(9) conidia/g polished rice to 18.2 x 10(9) conidia/g polished rice following application of these optimized conditions.

Keyword

Aerial conidia; Lecanicillium lecanii; Mycoinsecticide; Optimum condition; Solid-state fermentation

MeSH Terms

Aphids
Fermentation
Fungi
Humidity
Hydrogen-Ion Concentration
Seeds
Spores, Fungal
Steam
Steam