Mycobiology.  2010 Sep;38(3):210-214. 10.4489/MYCO.2010.38.3.210.

Selection of Lecanicillium Strain with High Virulence against Developmental Stages of Bemisia tabaci

• Park H
• Kim K

• KMID: 1457459
• DOI: http://doi.org/10.4489/MYCO.2010.38.3.210

Abstract

Selection of fungal strains with high virulence against the developmental stages of Bemisia tabaci was performed using internal transcribed spacer regions. The growth rate of hyphae was measured and bioassay of each developmental stage of B. tabaci was conducted for seven days. All of the fungal strains tested were identified as Lecanicillium spp., with strain 4078 showing the fastest mycelium growth rate (colony diameter, 16.3 +/- 0.9 mm) among the strains. Compared to strain 4075, which showed the slowest growth rate, the growth rate of strain 4078 was increased almost 2-fold after seven days. Strains 4078 and Btab01 were most virulent against the egg and larva stages, respectively. The virulence of fungal strains against the adult stage was high, except for strains 41185 and 3387. Based on the growth rate of mycelium and level of virulence, strains 4078 and Btab01 were selected as the best fungal strains for application to B. tabaci, regardless of developmental stage.

Keyword

Bemisia tabaci; Developmental stage; Internal transcribed spacer; Lecanicillium sp.; Virulence

MeSH Terms

Adult
Biological Assay
Humans
Hyphae
Larva
Mycelium
Ovum
Sprains and Strains