Mycobiology.  2010 Jun;38(2):137-143.

Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide

• Pham TA
• Kim JJ
• Kim K

Abstract

The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25degrees C culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 107 conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.

Keyword

Beauveria bassiana; Conidia production; Optimal culture conditions; Solid-state fermentation

MeSH Terms

Aphids
Beauveria
Fermentation
Humans
Meals
Polyethylene
Seeds
Spores, Fungal
Zea mays
Polyethylene