Mycobiology.  2008 Sep;36(3):178-182. 10.4489/MYCO.2008.36.3.178.

Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides

• Lee JW
• Baek SJ
• Kim YS

• KMID: 1487340
• DOI: http://doi.org/10.4489/MYCO.2008.36.3.178

Abstract

In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4.7H2O, 0.50 g/l; FeCl2.62O, 0.01 g/l; MnCl2.4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4.7H2O, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28degrees C; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

Keyword

Cultural condition; Optimal media; Phellinus linteus; Polysaccharide

MeSH Terms

Biomass
Bioreactors
Dihydroergotamine
Fructose
Hydrogen-Ion Concentration
Phosphates
Polysaccharides
Potassium Compounds
Yeasts
Dihydroergotamine
Fructose
Phosphates
Polysaccharides
Potassium Compounds