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Current Progress in Tendon and Ligament Tissue Engineering

Lim WL, Liau LL, Ng MH, Chowdhury , Law JX

BACKGROUND: Tendon and ligament injuries accounted for 30% of all musculoskeletal consultations with 4 million new incidences worldwide each year and thus imposed a significant burden to the society and...
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Histological Method to Study the Effect of Shear Stress on Cell Proliferation and Tissue Morphology in a Bioreactor

Chabanon M, Duval H, Grenier J, Beauchesne C, Goyeau B, David B

BACKGROUND: Tissue engineering represents a promising approach for the production of bone substitutes. The use of perfusion bioreactors for the culture of bone-forming cells on a three-dimensional porous scaffold resolves...
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Bioreactor systems are essentially required for stem cell bioprocessing

Cha JM, Lee MY, Hong J

As stem cell technologies have rapidly advanced, the stem cell therapy market has been forecast to reach hundreds of millions of USD in market value within the next 5 years....
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Tissue Regeneration of Human Mesenchymal Stem Cells on Porous Gelatin Micro-Carriers by Long-Term Dynamic In Vitro Culture

Nguyen L, Bang S, Noh I

BACKGROUND: Tissue engineering is a multidisciplinary field which attracted much attention in recent years. One of the most important issue in tissue engineering is how to obtain high cell numbers...
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Glucosamine Hydrochloride and N-Acetylglucosamine Influence the Response of Bovine Chondrocytes to TGF-β3 and IGF in Monolayer and Three-Dimensional Tissue Culture

Pizzolatti ALA, Gaudig F, Seitz D, Roesler CR, Salmoria G

BACKGROUND: Glucosamine hydrochloride (GlcN·HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical...
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Full Thickness Skin Expansion ex vivo in a Newly Developed Reactor and Evaluation of Auto-Grafting Efficiency of the Expanded Skin Using Yucatan Pig Model

Huh MI, Yi1 SJ, Lee KP, Kim HK, An SH, Kim DB, Ryu RH, Kim JS, Lim JO

BACKGROUND: Skin grafts are required in numerous clinical procedures, such as reconstruction after skin removal and correction of contracture or scarring after severe skin loss caused by burns, accidents, and...
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Production of Recombinant Anti-Cancer Vaccines in Plants

Lee JH, Ko K

Plant expression systems have been developed to produce anti-cancer vaccines. Plants have several advantages as bioreactors for the production of subunit vaccines: they are considered safe, and may be used...
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The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells

Lee JM, Kim MG, Byun JH, Kim GC, Ro JH, Hwang DS, Choi BB, Park GC, Kim UK

BACKGROUND: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor. METHODS: Human periosteal-derived stem cells were harvested...
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Prefabrication of Axially Vascularized Bone by Combining β-Tricalciumphosphate, Arteriovenous Loop, and Cell Sheet Technique

Ma D, Ren L, Cao Z, Li J, Cao J, Tian W, Yao H

The repair of bone defects poses a great challenge for reconstructive surgeons. Although the development of tissue engineering has exhibited promise in replacing damaged bone, the fabrication of large constructs...
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Bioreactor Conditioning of Valve Scaffolds Seeded Internally with Adult Stem Cells

Kennamer A, Sierad L, Pascal R, Rierson N, Albers C, Harpa M, Cotoi O, Harceaga L, Olah P, Terezia P, Simionescu A, Simionescu D

The goal of this study was to test the hypothesis that stem cells, as a response to valve-specific extracellular matrix “niches” and mechanical stimuli, would differentiate into valvular interstitial cells...
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The Effect of Polyurethane Scaffold Surface Treatments on the Adhesion of Chondrocytes Subjected to Interstitial Perfusion Culture

Raimondi M, Bertoldi S, Caddeo S, Farè S, Arrigoni C, Moretti M

The purpose of this study was to measure chondrocytes detachment from cellularized constructs cultured in a perfusion bioreactor, and to evaluate the effect of different scaffold coatings on cell adhesion...
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Rapid Expansion and Auto-Grafting Efficiency of Porcine Full Skin Expanded by a Skin Bioreactor Ex Vivo

Huh MI, An SH, Kim HG, Song YJ, Choi EC, An SH, Choi WS, Huh JS, Lim JO

Full skin auto-grafts are required for reconstruction of skin burns and trauma scars. However, currently available clinical approaches such as sheet skin graft, mesh skin grafts, artificial skin graft, and...
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Recent Advances for Enhancing Drug Metabolizing Functions of Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells

Han J, Kim JH

Hepatocyte-like cells (HLCs) derived from human pluripotent stem cells are a promising cell source for drug screening and toxicity tests. Thus, various hepatic differentiating protocols have been developed, leading to...
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Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial

Jeong C, Chung HY, Lim HJ, Lee JW, Choi KY, Yang JD, Cho BC, Lim JO, Yoo JJ, Lee SJ, Atala AJ

BACKGROUND: Tissue expansion is an effective and valuable technique for the reconstruction of large skin lesions and scars. This study aimed to evaluate the applicability and safety of a newly...
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Central Nervous System and the Colonic Bioreactor: Analysis of Colonic Microbiota in Patients with Stroke Unravels Unknown Mechanisms of the Host Defense after Brain Injury

Swidsinski A, Loening-Baucke V, Kruger M, Kirsch S

BACKGROUND/AIMS: Stroke is accompanied by septic complications due to quickly changing polymicrobials of unclear origin. This study was aimed to find the source of stroke-associated-infections. METHODS: We investigated the biostructure of...
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Effects of combined mechanical stimulation on the proliferation and differentiation of pre-osteoblasts

Kang KS, Lee SJ, Lee H, Moon W, Cho DW

We observed how combined mechanical stimuli affect the proliferation and differentiation of pre-osteoblasts. For this research, a bioreactor system was developed that can simultaneously stimulate cells with cyclic strain and...
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Digestion Pattern of Antihypertensive Angiotensin I-Converting Enzyme Inhibitory Peptides from Saccharomyces cerevisiae in a Successive Simulated Gastricintestinal Bioreactor

Jang JH, Jeong SC, Lee JK, Lee JS

  • KMID: 2312617
  • Mycobiology.
  • 2011 Mar;39(1):67-69.
A cell-free extract of Saccharomyces cerevisiae containing the angiotensin I-converting enzyme (ACE) inhibitory peptide was treated in a successive simulated gastric-intestinal bioreactor (step 1: amylase digestion, step 2: gastric fluid...
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Artificial Liver Devices and Bioartificial Liver Systems: Current Status

Kwon CH, Lee SK, Park JK, Lee DH, Lee JH

Acute liver failure is a rapidly progressive disease of the liver associated with high morbidity and mortality without liver transplantation. Although good survival after transplantation can be achieved, due to...
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Effects of Mechanical Stimulation and Scaffolds for Nucleus Pulposus Replacement of Intervertebral Disc

Ha SH, Kim DH, Shin JW, Gwak HC, Choi JS

  • KMID: 2199044
  • J Korean Orthop Res Soc.
  • 2009 Dec;12(2):45-52.
PURPOSE: In this study, we investigated the potential of injectable hydrogel scaffolds for the regeneration of nucleus pulposus. MATERIALS AND METHODS: We prepared injectable hydrogels [Chitosan-Pluronic (CP), CP/Osteogenic Protein-1 (CP/OP-1), CP/Gly-Arg-Gly-Asp-Ser...
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Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides

Lee JW, Baek SJ, Kim YS

In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium...
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