J Bacteriol Virol.  2008 Dec;38(4):189-196. 10.4167/jbv.2008.38.4.189.

Detection of Virulence Genes of Staphyloccus aureus and Staphylococcus epidermidis Isolated from Suprapubic Urine from Infants with Fever

Affiliations
  • 1Department of Microbiology, School of Medicine, Ewha Womans University, Seoul, Korea. parkhk@ewha.ac.kr
  • 2Deprtment of Microbiology, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea.
  • 3Department of Preventive Medicine, and 4Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul, Korea.

Abstract

While methicillin-resistant Staphylococcus aureus (MRSA) isolated from urinary tract infection (UTI) has recently increased in elderly adult urology patients, it has been only rarely reported in infants. Therefore, in this study to understand whether MRSA may be involved in UTI of infants who run fever without other apparent causes, we identified and counted S. aureus and S. epidermidis in suprapubic urine from 750 febrile infants via microbiological methods, and confirmed the counts via multiplex PCR. And we also detected four virulence genes, mecA, PVL, bbp and icaA genes for S. aureus and S. epidermidis via multiplex PCR in the same specimens. S. aureus (28 cases) counts were as follows: >10(4) CFU/ml (3/28), 10(2)~10(3) CFU/ml (1/28) and <10(2)~10(3) CFU/ml (24/28). S. epidermidis (26 cases) counts were as follows: >10(4) CFU/ml (2/26), 10(2)~10(3) CFU/ml (4/26) and 10(2)~10(3) CFU/ml (20/26). S. aureus virulence genes were detected in 26 cases as mecA (16/26, 59.3%), PVL (17/26, 63.0%), bbp (7/26, 26.9%) and icaA (20/26, 76.9%). S. epidermidis virulence genes were detected in 22 cases as mecA (17/22, 81.0%), PVL (15/22, 71.4%), bbp (3/22, 13.6%) and icaA (13/22, 50.1%). Therefore, mecA, PVL and icaA genes of MRSA and MRSE were detected with high positivity in urines from infants with fever. The results demonstrate that community-acquired MRSA or MRSE may be responsible for UTI incidence in febrile infants.

Keyword

Suprapubic urine; Staphylococcus aureus; Staphyococcus epidermidis; mecA; PVL; bbp; icaA

MeSH Terms

Adenosine
Adult
Aged
Benzophenones
Boronic Acids
Fever
Humans
Incidence
Infant
Methicillin-Resistant Staphylococcus aureus
Multiplex Polymerase Chain Reaction
Staphylococcus
Staphylococcus aureus
Staphylococcus epidermidis
Urinary Tract Infections
Urology
Adenosine
Benzophenones
Boronic Acids
Full Text Links
  • JBV
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error