J Bacteriol Virol.  2008 Dec;38(4):189-196. 10.4167/jbv.2008.38.4.189.

Detection of Virulence Genes of Staphyloccus aureus and Staphylococcus epidermidis Isolated from Suprapubic Urine from Infants with Fever

• Park HK
• Woo SY
• Jung YJ
• Lee EO
• Cha JE
• Park HS
• Lee SJ

• KMID: 1483959
• DOI: http://doi.org/10.4167/jbv.2008.38.4.189

Abstract

While methicillin-resistant Staphylococcus aureus (MRSA) isolated from urinary tract infection (UTI) has recently increased in elderly adult urology patients, it has been only rarely reported in infants. Therefore, in this study to understand whether MRSA may be involved in UTI of infants who run fever without other apparent causes, we identified and counted S. aureus and S. epidermidis in suprapubic urine from 750 febrile infants via microbiological methods, and confirmed the counts via multiplex PCR. And we also detected four virulence genes, mecA, PVL, bbp and icaA genes for S. aureus and S. epidermidis via multiplex PCR in the same specimens. S. aureus (28 cases) counts were as follows: >10(4) CFU/ml (3/28), 10(2)~10(3) CFU/ml (1/28) and <10(2)~10(3) CFU/ml (24/28). S. epidermidis (26 cases) counts were as follows: >10(4) CFU/ml (2/26), 10(2)~10(3) CFU/ml (4/26) and 10(2)~10(3) CFU/ml (20/26). S. aureus virulence genes were detected in 26 cases as mecA (16/26, 59.3%), PVL (17/26, 63.0%), bbp (7/26, 26.9%) and icaA (20/26, 76.9%). S. epidermidis virulence genes were detected in 22 cases as mecA (17/22, 81.0%), PVL (15/22, 71.4%), bbp (3/22, 13.6%) and icaA (13/22, 50.1%). Therefore, mecA, PVL and icaA genes of MRSA and MRSE were detected with high positivity in urines from infants with fever. The results demonstrate that community-acquired MRSA or MRSE may be responsible for UTI incidence in febrile infants.

Keyword

Suprapubic urine; Staphylococcus aureus; Staphyococcus epidermidis; mecA; PVL; bbp; icaA

MeSH Terms

Adenosine
Adult
Aged
Benzophenones
Boronic Acids
Fever
Humans
Incidence
Infant
Methicillin-Resistant Staphylococcus aureus
Multiplex Polymerase Chain Reaction
Staphylococcus
Staphylococcus aureus
Staphylococcus epidermidis
Urinary Tract Infections
Urology
Adenosine
Benzophenones
Boronic Acids

Figure

• 

  Figure 1. S. aureus and S. epidermidis isolated from the suprapubic urines of infants were confirmed via multiplex PCR. Lane 1 = DNA marker (100 bp ladder); lane 2 = positive control for S. aureus (108 bp); lane 3 = S. aureus positive sample; lane 4 = positive control for S. epidermidis (125 bp); lane 5 = S. epidermidis positive sample; lane 6 = DNA marker; lane 7 = S. epidermidis positive; lane 8 and 9 = S. aureus positive; lane 10 = S. aureus and mecA positive; lane 11 = S. epidermidis and mecA positive; lane 12 = DNA marker.

• 

  Figure 2. mecA gene and PVL gene were detected via multiplex PCR from S. aureus and S. epidermidis isolated from the suprapubic urines of infants. Lane 1 = positive control for mecA gene (310 bp); lane 2 = positive control for PVL gene (433 bp); lane 3 = mecA positive sample; lane 4 = PVL positive samlple; lane 5 = mecA and PVL positive; lane 6 = DNA marker (100 bp ladder); lane 7 = mecA and PVL positive; lane 8 = PVL positive; lane 9 = mecA and PVL positive; lane 10 = negative control for mecA and PVL gene; lane 11 = mecA and PVL negative sample; lane 12 = DNA marker.

• 

  Figure 3. bbp gene and icaA gene were detected via multiplex PCR from S. aureus and S. epidermidis isolated from the suprapubic urines of infants. Lane 1 = DNA marker (100 bp ladder); lane 2 = positive control for bbp gene (575 bp); lane 3 = bbp and icaA positive sample; lane 4, 5, and 6 = icaA positive; lane 7 = DNA marker (100 bp ladder); lane 8 = positive control for icaA (103 bp); lane 9 = negative control for icaA; lane 10 = negative control for bbp; lane 11 and 12 = icaA positive; lane 13 = DNA marker.

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