Korean J Nephrol.
2009 May;28(3):180-189.
The Effect of Extract of Paeonia lactiflora on the Improvement of Ischemic Acute Renal Failure
- Affiliations
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- 1Department of Biochemistry, School of Medicine Catholic University of Daegu, Daegu, Korea. leejw@cu.ac.kr
- 2Department of Internal Medicine, School of Medicine Catholic University of Daegu, Daegu, Korea.
- 3Hypoxi Co., Ltd., Daegu, Korea.
- 4Gyeongbuk Agricultural Technology Administration, Gyeongbuk , Korea.
- 5Natural Medicines Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Abstract
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PURPOSE:The present study was undertaken to investigate whether the extract or the ethyl acetate fraction of Paeonia lactiflora, which improves cell survival under ischemic condition by inhibiting apoptosis, can prevent ischemic acute renal failure, using rats as an animal model.
METHODS
In the control group, ischemia/reperfusion (I/R) injury was induced in male Sprague-Dawley rats by clamping of left renal pedicle for 45 minutes after removal of the right kidney, and subsequent reperfusion of the pedicle for 24 hours. In the experimental group, the water extract or ethyl acetate fraction of methanol extract Paeonia lactiflora was injected 1 hour prior to ischemia/reperfusion injury. We measured serum concentrations of creatinine at 24 hours after I/R injury. And the kidneys were extracted, fixed in a 10 % neutral-buffered formalin solution, embedded in paraffin and used for histopathological examination.
RESULTS
Rats in the experimental group, treated with water extract or ethyl acetate fraction of methanol extract of Paeonia lactiflora, exhibited significant decrease in the serum concentrations of creatinine (approx. 2.0 mg/dL), compared with those in the control group (approx. 4.0 mg/dL). Finally, the cell morphology of the kidney of rats treated with ethyl acetate fraction of Paeonia lactiflora was well preserved, when judged from histopathological point of view.
CONCLUSION
Pretreatment of rats with the water extract or ethyl acetate fraction of Paeonia lactiflora might be beneficial for the treatment of acute renal failure in humans.
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