J Korean Soc Ther Radiol Oncol.  2010 Jun;28(2):91-98. 10.3857/jkstro.2010.28.2.91.

The Effect of Troglitazone on Thermal Sensitivity in Uterine Cervix Cancer Cells

• Lee JH
• Kim WD
• Yu JR
• Park WY

• KMID: 1460157
• DOI: http://doi.org/10.3857/jkstro.2010.28.2.91

Abstract

PURPOSE
Troglitazone (TRO), a PPAR-gamma agonist, can reduce heat shock protein (HSP) 70 and increase the antioxidant enzymes, such as superoxide dismutase (SOD) and catalase, which might affect thermal sensitivity. Here, we investigated whether TRO modifies thermal sensitivity in uterine cervical cancer cells, which is most commonly treated by hyperthermia (HT).
MATERIALS AND METHODS
HeLa cells were treated with 5microM TRO for 24 hours before HT at 42degrees C for 1 hour. Cell survival was analyzed by clonogenic assay. The expression of HSPs was analyzed by Western blot. SOD and catalase activity was measured and reactive oxygen species (ROS) was measured using 2',7'-dichlorofluorescin diacetate and dihydroethidium.
RESULTS
The decreased cell survival by HT was increased by preincubation with TRO before HT. Expression of HSP 70 was increased by HT however, it was not decreased by preincubation with TRO before HT. The decreased Bcl-2 expression by HT was increased by preincubation with TRO. SOD and catalase activity was increased by 1.2 and 1.3 times,respectively with TRO. Increased ROS by HT was decreased by preincubation with TRO.
CONCLUSION
TRO decreases thermal sensitivity through increased SOD and catalase activity, as well as scavenging ROS in HeLa cells.

Keyword

Troglitazone; Hyperthermia; Reactive oxygen species

MeSH Terms

Blotting, Western
Catalase
Cell Survival
Cervix Uteri
Chromans
Ethidium
Female
Fever
Fluoresceins
Heat-Shock Proteins
HeLa Cells
Humans
Reactive Oxygen Species
Superoxide Dismutase
Thiazolidinediones
Uterine Cervical Neoplasms
Catalase
Chromans
Ethidium
Fluoresceins
Heat-Shock Proteins
Reactive Oxygen Species
Superoxide Dismutase
Thiazolidinediones