Lab Anim Res.  2010 Mar;26(1):21-29. 10.5625/lar.2010.26.1.21.

In vitro and in vivo Antibacterial Activities of Cinnamomum cassia Extracts Against Helicobacter pylori

• Lee HA
• Hong S
• Oh HG
• Park SH
• Kim YC
• Jeong GS
• Kim O

• KMID: 1458936
• DOI: http://doi.org/10.5625/lar.2010.26.1.21

Abstract

Infection with Helicobacter pylori (H. pylori) is strongly associated with duodenal and gastric ulcers. Substantial epidemiological data has revealed that high rates of H. pylori infection might be related to high rates of gastric cancer and gastric adenocarcinoma. In this study, a medicinal herbal plant, Cinnamomum cassia, was examined and screened for anti-H. pylori activity. Seventy percent ethanol was used for herbal extraction. For anti-H. pylori activity screening, inhibitory zone tests as an in vitro assay and in vivo study using a Mongolian gerbil (Meriones unguiculatus) model were performed. Also, the safety of herbal compounds was evaluated by animal study. As a result of inhibitory zone test, Cinnamomum cassia extract demonstrated strong anti-H. pylori activities. Also, as results of in vivo animal studies, Cinnamomum cassia demonstrated strong therapeutic effects against H. pylori infection according to the criteria of histological examination and rapid urease test. As results of the safety study, after 28 days treatment of the Cinnamomum cassia extract, the animals were not detected any grossly and histological changes. These results demonstrate that it can be successfully cured against H. pylori infection and protected from H. pylori-induced pathology with Cinnamomum cassia. It could be a promising native herb treatment for patients with gastric complaints including gastric ulcer caused by H. pylori.

Keyword

Cinnamomum cassia; Helicobacter pylori; antibacterial activity; Mongolian gerbil

MeSH Terms

Adenocarcinoma
Animals
Cinnamomum
Cinnamomum aromaticum
Ethanol
Gerbillinae
Helicobacter
Helicobacter pylori
Humans
Mass Screening
Plants
Plants, Medicinal
Stomach Neoplasms
Stomach Ulcer
Urease
Ethanol
Urease

Figure

• 

  Figure 1. The anti-H. pylori activities of Cinnamomum cassia extract using the paper disk diffusion bioassay. The diameter of clear zone reveals anti-H. pylori activities of the inoculated materiel. C. cassia (100): Cinnamomum cassia extract 1.5 mg, C. cassia (10−1): Cinnamomum cassia extract 0.15 mg.

• 

  Figure 2. Gross findings of the stomach in the study on therapeutic effects of H. pylori infection with Cinnamomum cassia extract. A: H. pylori+Cinnamomum cassia, B: H. pylori+ PBS (black arrow: ulcer lesions), C: PBS+Cinnamomum cassia, D: PBS+PBS.

• 

  Figure 3. Histopathological findings of the stomach in the study on therapeutic effects of H. pylori infection with Cinnamomum cassia extract. A: H. pylori+Cinnamomum cassia, B: H. pylori+ PBS (Cellular degeneration and atrophy in the mucous layer), C: PBS+Cinnamomum cassia, D: PBS+PBS.

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