Korean J Pathol.  2012 Feb;46(1):30-37. 10.4132/KoreanJPathol.2012.46.1.30.

TNF-alpha and TNF-beta Polymorphisms are Associated with Susceptibility to Osteoarthritis in a Korean Population

Affiliations
  • 1Department of Pathology, The Catholic University of Korea College of Medicine, Seoul, Korea. wonsang@catholic.ac.kr
  • 2Department of Orthopedics, The Catholic University of Korea College of Medicine, Seoul, Korea.
  • 3Department of Biostatistics, The Catholic University of Korea College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
The tumor necrosis factor (TNF) is believed to play an important role in the pathophysiology of osteoarthritis (OA). Evidence shows that genetic polymorphisms make substantial contributions to the etiology of OA.
METHODS
We investigated the genotypes TNF-alpha and TNF-beta in 301 OA patients and 291 healthy subjects as controls. We employed a polymerase chain reaction-restriction fragment length polymorphism and a polymerase chain reaction-single strand conformation polymorphism assay to identify the genotypes TNFA -G308A and TNFB +G252A, respectively.
RESULTS
For TNFA -G308A, the percentages of genotypes GG, AG, and AA were 26.3% (79/301), 62.5% (188/301), and 11.3% (34/301) in OA patients and 88.7% (258/291), 11.3% (33/291), and 0% (0/291) in controls. For TNFB +G252A, the percentages of genotypes GG, AG, and AA were 15.3% (46/301), 41.9% (126/301), and 42.9% (129/301) in OA patients and 12% (35/291), 52.6% (153/291), and 35.4% (103/291) in controls. There were significant differences in genotypes and alleles of TNFA -308 between OA patients and controls (p<0.0001) and in alleles of TNFB +252 (p=0.0325). The risk of OA was significantly higher for carriers of the TNFA -308A allele and the TNFB +252 AA homozygote (p=0.0224).
CONCLUSIONS
The results suggest close relationships between TNFA -G308A and TNFB +G252A polymorphisms and individual susceptibility to OA in the Korean population.

Keyword

Genetic polymorphism; Genetic predisposition to disease; Osteoarthritis; Tumor necrosis factor-alpha; Lymphotoxin-alpha

MeSH Terms

Alleles
Genetic Predisposition to Disease
Genotype
Homozygote
Humans
Lymphotoxin-alpha
Osteoarthritis
Polymorphism, Genetic
Tumor Necrosis Factor-alpha
Lymphotoxin-alpha
Tumor Necrosis Factor-alpha
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error