Korean J Pediatr.  2009 Apr;52(4):476-480. 10.3345/kjp.2009.52.4.476.

Polymorphisms of tumor necrosis factor-alpha promotor gene in Kawasaki disease and relation to the risk of coronary artery lesion

• Kim SH
• Yun JW
• Lee YH
• Cheon EJ

• KMID: 2281749
• DOI: http://doi.org/10.3345/kjp.2009.52.4.476

Abstract

PURPOSE
The purpose of this study was to investigate the polymorphisms of the TNF-alpha promotor gene, its susceptibility to Kawasaki disease (KD) and to assess whether the TNF-alpha promotor gene polymorphism was related the risk of coronary artery lesions (CALs). METHODS: From January 2003 to January 2007, 51 children (30 boys and 21 girls) with KD and 48 children forming an age-matched control group were studied. DNA from the peripheral blood of all the children was sampled, and the DNA polymorphisms of the 5' flanking regions of the TNF-alpha promoter gene at position -308 [guanine (G) to adenine (A)] were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Then, the relationship between KD and the TNF-alpha promotor gene polymorphisms was evaluated. RESULTS: The A allele frequency of the -308 site of the TNF-alpha promotor gene was 17.6% (9/51) for children with KD and 6.8% (3/48) for the control group children, but this result was not statistically significant. Twenty-four patients experienced CALs within 60 days after the onset of symptoms. KD children with TNF-alpha -308 A allele had lower frequencies of CALs (12.5% versus 22.2%, P>0.05). CONCLUSIONS: The DNA polymorphism of the -308 site TNF-alpha gene was not associated with susceptibility to KD and a risk of CALs. Multicenter, large-scale randomized controlled trials are needed for further study.

Keyword

Mucocutaneous lymph node syndrome; Coronary arteries; TNF-alpha polymorphism

MeSH Terms

5' Flanking Region
Adenine
Alleles
Child
Coronary Vessels
DNA
Gene Frequency
Humans
Mucocutaneous Lymph Node Syndrome
Tumor Necrosis Factor-alpha
Adenine
DNA
Tumor Necrosis Factor-alpha