Korean J Pediatr Infect Dis.  2012 Aug;19(2):71-78.

Phylogenetic Analysis of Human Bocavirus in Hospitalized Children with Acute Respiratory Tract Infection in Korea

• Ahn JG
• Choi SY
• Kim DS
• Kim KH

Abstract

PURPOSE
Human bocavirus (hBoV), a recently discovered virus, has been detected in children with respiratory tract infections worldwide. The aim of this study was to analyze the frequency and molecular phylogeny of hBoV in the respiratory samples of children with acute respiratory tract infections in 2010.
METHODS
Nasopharyngeal samples were collected from 953 children with lower respiratory tract infections at Severance children's hospital in Korea from January 2010 to December 2010. We applied the multiplex PCR technique for the identification of 12 respiratory viruses from the samples. Among the total specimens, hBoV positive samples were subjected to phylogenetic analysis by sequencing a fragment of the VP1/VP2 gene junction.
RESULTS
hBoV was detected in 141 (14.8%) among 953 patients. The 61.7% of hBoV-positive samples were found to co-exist with other respiratory viruses. The results of phylogenetic analysis showed that all 141 hBoV-positive isolates were identified as hBoV 1, revealing a high similarity among the isolates (>98%).
CONCLUSION
hBoV 1 with minimal sequence variations circulated in children with acute respiratory infections during 2010. More research is needed to determine the clinical severity and outcomes of the minimal sequence variations.

Keyword

Human bocavirus; Acute respiratory infections; Phylogenetic analysis

MeSH Terms

Child
Child, Hospitalized
Human bocavirus
Humans
Korea
Multiplex Polymerase Chain Reaction
Phylogeny
Respiratory System
Respiratory Tract Infections
Viruses

Figure

• 

  Fig. 1 Distribution of identified respiratory viruses. Abbreviations: ADV, adenovirus; hRV, human rhinovirus; RSV, respiratory syncytial virus; hBoV, human bocavirus; hMPV, human metapneumovirus; IFB, influenza virus type B; PIV, parainfluenza virus; hCoV, human coronavirus.

• 

  Fig. 2 Phylogenetic analysis of the VP1/VP2 gene junction sequences in hBoV isolates. The topology tree was constructed by the neighbor-joining method using MEGA 5.0. Black dots designate reference strains, the other were sequences analyzed in the present study. GenBank accession no. HBoV2 PK-5510 (FJ170278); HBoV3 W47 1 (EU918736); HBoV4-NI-385 (FJ973561); HK24 (EF4 50740); LWK (GU338055); TW2715_06 (EU984232); CU49 (EF203921; st1 (HBoVstrain st1, DQ000495); HK11 (EF450727); CZ643 (DQ457413); CRD2 (DQ34 0570); st2 (HBoV strain st2, DQ000496).

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