Korean J Vet Res.
2012 Jun;52(2):125-131.
Sequential pathologic changes and viral distribution in rabbits experimentally infected with new Korean strain of rabbit hemorrhagic disease virus (RHDVa)
- Affiliations
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- 1Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea.
- 2Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul 120-749, Korea.
- 3Department of Animal Science, College of Life Science and Natural Resources, Sangji University, Wonju 220-702, Korea.
- 4Department College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
- 5Department of Clinical Laboratory Science, Semyung University, Jecheon 390-711, Korea. ghwoo@naver.com
Abstract
- Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.
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