J Korean Assoc Maxillofac Plast Reconstr Surg.  2012 Mar;34(2):91-99.

Osteogenic Gene Expression on Anodizing Titanium Surface

• Kim W
• Kim YS
• Jeon S
• Jun S
• Lee E
• Jang H
• Kwon J
• Rim J

Abstract

PURPOSE
The purpose of this study was to evaluate the expression of osteogenic genes associated with bone regeneration on anodizing titanium surface.
METHODS
20x20x1 (mm) commercially pure titanium plate was made, one group was pure titanium, second group was punched, and last group was punched and anodized by electrochemical method. Through the osteogenic cell culture model, the expression of extracellular matrix proteins, such as bone morphogenetic protein-2, bone sialoprotein, aggrecan, osteocalcin, Alkaline phosphatase, collagen I had been evaluated by Real-time polymerase chain reaction, and the morphology of growing cells was evaluated by scanning electron microscopy.
RESULTS
The attachment of mesenchymal stem cell was even and well-oriented on all Ti surfaces. The osteogene expression was increased on punching groups but, decreased on anodizing surfaces in 3 week samples.
CONCLUSION
Punched anodizing Ti has possibility be using as a dental implant material, but further in vivo study would be needed.

Keyword

Anodized titanium; Osteogenic genes; Pulp stem cells; Dental implants

MeSH Terms

Aggrecans
Alkaline Phosphatase
Bone Regeneration
Cell Culture Techniques
Collagen
Dental Implants
Durapatite
Electrons
Extracellular Matrix Proteins
Gene Expression
Integrin-Binding Sialoprotein
Mesenchymal Stromal Cells
Osteocalcin
Real-Time Polymerase Chain Reaction
Titanium
Aggrecans
Alkaline Phosphatase
Collagen
Dental Implants
Durapatite
Extracellular Matrix Proteins
Integrin-Binding Sialoprotein
Osteocalcin
Titanium