Korean J Vet Res.
2012 Dec;52(4):231-238.
Cytokine expression pattern in milk somatic cells of subclinical mastitis-affected cattle analyzed by real time PCR
- Affiliations
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- 1Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, Anand 388120, Gujarat, India. bhatt_vbhv@yahoo.co.in
- 2Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India.
- 3Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India.
- 4Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India.
Abstract
- The expression profiles of inflammatory cytokines viz. interleukins (IL)-6, IL-8, IL-12, granulocyte macrophage-colony stimulating factor, interferon-gamma and tumor necrosis factor-alpha in response to subclinical mastitis in indigenous cattle breed Kankrej (n = 6), Gir (Bos indicus) (n = 12) and crossbred (Bos taurus x Bos indicus) (n = 7) were investigated using quantitative real time PCR. Significant correlation (p < 0.05) was observed between total bacterial load and somatic cell count (SCC) in all three breeds of cattle. All the cytokines were observed to be up-regulated compared to cows with healthy quarters, however, level of their expression varied among three breeds of cattle. In Kankrej most cytokines were found to be transcribed to higher levels than in other two breeds; the milk had higher load of bacteria but not so high SCC, implying that Kankrej has a higher inherent resistance against mastitis. The results of present study indicated that mammary glands of crossbred cattle are more sensitive to bacterial infection than indigenous breed of cattle as they elicit immune response at lower bacterial load and result into higher SCC. Research on identification of factors responsible for differentially expressed cytokines profiles and use of cytokines as immunomodulatory tools can pave way for formulating control strategies against bovine mastitis.
MeSH Terms
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Animals
Bacteria
Bacterial Infections
Bacterial Load
Cattle
Cell Count
Cytokines
Female
Granulocytes
Interferon-gamma
Interferons
Interleukin-12
Interleukin-8
Interleukins
Mammary Glands, Human
Mastitis
Mastitis, Bovine
Milk
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Cytokines
Interferon-gamma
Interferons
Interleukin-12
Interleukin-8
Interleukins
Tumor Necrosis Factor-alpha
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