Clin Exp Reprod Med.  2013 Mar;40(1):23-28. 10.5653/cerm.2013.40.1.23.

The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis

• Kim HS
• Kang MJ
• Kim SA
• Oh SK
• Kim H
• Ku SY
• Kim SH
• Moon SY
• Choi YM

• KMID: 1430079
• DOI: http://doi.org/10.5653/cerm.2013.40.1.23

Abstract

OBJECTIVE
The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation.
METHODS
Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests.
RESULTS
Prevalence of strict morphology of less than 4%, and abnormal sperm chromatin structure and condensation did not show any statistically significant differences according to male age (p=0.605, p=0.235, and p=0.080). No significant correlation was demonstrated among age of male partners, strict morphology, and abnormal sperm chromatin structure using TB and AB tests. However, abnormal sperm chromatin condensation was positively associated with sperm chromatin structure (r=0.594, p=0.000) and showed negative correlation with strict morphology (r=-0.219, p=0.029).
CONCLUSION
The tests for sperm chromatin condensation showed a significant association with strict morphology. Further study is needed to elucidate the relationship between clinical outcome and sperm chromatin tests.

Keyword

Toluidine blue; Aniline blue; Semen analysis; DNA damage; Human

MeSH Terms

Aniline Compounds
Chromatin
DNA
DNA Damage
Humans
Male
Prevalence
Semen
Semen Analysis
Spermatozoa
Tolonium Chloride
Aniline Compounds
Chromatin
DNA
Tolonium Chloride