Exp Mol Med.  2004 Apr;36(2):130-134.

Nerve growth factor(NGF) induces mRNA expression of the new transcription factor protein p48ZnF

• Heese K
• Nagai Y
• Sawada T

Abstract

Apoptosis, the cell's intrinsic death program, plays a crucial role in the regulation of tissue homeostasis, and abnormal inhibition of apoptosis is an indicator of cancer and autoimmune diseases, whereas excessive cell death is implicated in neurodegenerative disorders such as Alzheimer's disease (AD). Using cDNA subtraction analysis, we compared p60TRP (p60 transcription regulator protein) expressing cells with control cells during the process of apoptosis and we identified the new zinc-finger protein p48ZnF that is predominantly located in the cytoplasm of the cell. Additionally, we demonstrate here that p48ZnF is up-regulated in rat neuronal PC12 cells upon stimulation with the neurotrophic factor NGF (50 ng/ml). These findings point to a possible pivotal role of p48ZnF in the control of neuronal survival.

Keyword

Alzheimer's disease; apoptosis, neurodegeneration; neurotrophins; transcription

MeSH Terms

Alzheimer Disease/genetics/metabolism
Animals
Apoptosis
Autoimmune Diseases/genetics/metabolism
Base Sequence
Biological Markers
CHO Cells
Cell Survival/drug effects
Cloning, Molecular
Cytoplasm/*metabolism
DNA-Binding Proteins/*biosynthesis
Gene Expression Profiling
Gene Expression Regulation/drug effects
Hamsters
Molecular Sequence Data
Neoplasms/metabolism
Nerve Growth Factor/*pharmacology
Nerve Tissue Proteins/*biosynthesis
PC12 Cells
RNA, Messenger/*biosynthesis
Rats
Research Support, Non-U.S. Gov't
Transcription Factors/*biosynthesis/*genetics
Transfection
Zinc Fingers/*genetics