J Korean Med Sci.  2003 Oct;18(5):679-685. 10.3346/jkms.2003.18.5.679.

Production of Stromal Cell-Derived Factor-1 (SDF-1)and Expression of CXCR4 in Human Bone Marrow Endothelial Cells

• Yun HJ
• Jo DY

• KMID: 1130038
• DOI: http://doi.org/10.3346/jkms.2003.18.5.679

Abstract

This study investigated the production of stromal cell-derived factor-1 (SDF-1) and the expression of CXCR4 in human bone marrow endothelial cells (BMECs). Human BMEC cell line BMEC-1 cells expressed SDF-1 mRNA, and conditioned medium induced chemoattraction of CD34+ cells. Migration was not inhibited by pretreating the input cells with pertussis toxin, indicating that the chemoattractive activity was not dependent on SDF-1. Three-day culture of BMEC-1 and primary human BMEC cells produced 1,710+/-204 and 1,050+/-153 pg/mL SDF-1alpha, respectively, which was much less than primary human BM stromal cells (29,536+/-532 pg/ mL). By immuno-histochemistry, CXCR4 was detected in the endothelial cells lining sinusoids, arterioles, and venules in the bone marrow. However, cultured BMECs and BMEC-1 cells did not express CXCR4 on their surfaces. These results indicate that BMECs produce and release small amounts of SDF-1 and express CXCR4 in vivo only.

Keyword

Bone Marrow; Endothelium, Vascular; Chemokines, CXC; Receptors, CXCR4; Chemotaxis

MeSH Terms

Antigens, CD34/biosynthesis
Bone Marrow Cells/*metabolism
Cell Movement
Cells, Cultured
Chemokines, CXC/*biosynthesis
Chemotaxis
Culture Media, Conditioned/pharmacology
Endothelial Cells/*metabolism
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Hematopoietic Stem Cells/metabolism
Human
Immunohistochemistry
Pertussis Toxin/pharmacology
RNA, Messenger/metabolism
Receptors, CXCR4/*biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Support, Non-U.S. Gov't
Time Factors
Umbilical Veins/cytology