J Korean Med Sci.  2000 Jun;15(3):323-326. 10.3346/jkms.2000.15.3.323.

Effects of oncostatin M on hormone release of rat pituitary cells in primary culture

• Kim DS
• Choi HS
• Park YS
• Kim TW

• KMID: 1128632
• DOI: http://doi.org/10.3346/jkms.2000.15.3.323

Abstract

It has become increasingly clear that cytokines play an important role in modulating neuroendocrine regulation, especially in the secretion of corticotropin (ACTH) in the pituitary. Oncostatin M (OSM), a cytokine of IL-6 family has been reported to increase ACTH secretion and pro-opiomelanocortin (POMC) transcription in murine corticotroph pituitary tumor cells (AtT20 cells). The present study was undertaken to determine the effects of OSM on hormonal release in primary culture of rat pituitary cells. Growth hormone or prolactin release was not affected by OSM. OSM (1 nM) stimulated ACTH release (35.1% increase versus control, p>0.001) in dispersed pituitary cells of rat to a lesser extent than in AtT20 cells. Corticotropin releasing hormone (CRH) (10 nM) also induced a 2.3-fold increase of ACTH secretion (p>0.001), but co-treatment of OSM and CRH did not exhibit any synergistic effect on ACTH secretion. We conclude OSM has a stimulatory effect on ACTH secretion in normal rat pituitary cell cultures, and OSM acts mainly on corticotroph, supporting the potential role of OSM to modulate immune-endocrine regulation in the pituitary.

Keyword

Cytokines, Oncostatin M; Pituitary Gland; Corticotropin

MeSH Terms

Animal
Cells, Cultured
Corticotropin/secretion*
Cytokines/pharmacology
Cytokines/metabolism*
Inflammation Mediators/pharmacology
Inflammation Mediators/metabolism*
Male
Peptides/pharmacology
Peptides/metabolism*
Pituitary Gland/metabolism*
Pituitary Gland/drug effects
Pituitary Gland/cytology
Prolactin/secretion*
Rats
Rats, Inbred WF
Somatotropin/secretion*