Exp Mol Med.  2011 Oct;43(10):561-570. 10.3858/emm.2011.43.10.062.

Grape seed proanthocyanidin extract ameliorates monosodium iodoacetate-induced osteoarthritis

• Woo YJ
• Joo YB
• Jung YO
• Ju JH
• Cho ML
• Oh HJ
• Jhun JY
• Park MK
• Park JS
• Kang CM
• Sung MS
• Park SH
• Kim HY
• Min JK

• KMID: 1123368
• DOI: http://doi.org/10.3858/emm.2011.43.10.062

Abstract

Osteoarthritis (OA) is an age-related joint disease that is characterized by degeneration of articular cartilage and chronic pain. Oxidative stress is considered one of the pathophysiological factors in the progression of OA. We investigated the effects of grape seed proanthocyanidin extract (GSPE), which is an antioxidant, on monosodium iodoacetate (MIA)-induced arthritis of the knee joint of rat, which is an animal model of human OA. GSPE (100 mg/kg or 300 mg/kg) or saline was given orally three times per week for 4 weeks after the MIA injection. Pain was measured using the paw withdrawal latency (PWL), the paw withdrawal threshold (PWT) and the hind limb weight bearing ability. Joint damage was assessed using histological and microscopic analysis and microcomputerized tomography. Matrix metalloproteinase-13 (MMP13) and nitrotyrosine were detected using immunohistochemistry. Administration of GSPE to the MIA-treated rats significantly increased the PWL and PWT and this resulted in recovery of hind paw weight distribution (P < 0.05). GSPE reduced the loss of chondrocytes and proteoglycan, the production of MMP13, nitrotyrosine and IL-1beta and the formation of osteophytes, and it reduced the number of subchondral bone fractures in the MIA-treated rats. These results indicate that GSPE is antinociceptive and it is protective against joint damage in the MIA-treated rat model of OA. GSPE could open up novel avenues for the treatment of OA.

Keyword

antioxidants; grape seed proanthocyanidins; inflammation; interleukin-1beta; osteoarthritis

MeSH Terms

Analgesics/*administration & dosage
Animals
Antioxidants/*administration & dosage
Bone Resorption
Disease Models, Animal
Gene Expression Regulation
Humans
Interleukin-1beta/genetics/metabolism
Iodoacetates/administration & dosage
Knee Joint/*drug effects/metabolism/pathology
Male
Matrix Metalloproteinase 13/genetics/metabolism
Osteoarthritis/chemically induced/*drug therapy/physiopathology
Pain
Plant Extracts/administration & dosage
Proanthocyanidins/*administration & dosage
Rats
Rats, Wistar
Seeds
Tomography, Emission-Computed
Tyrosine/analogs & derivatives/metabolism
Vitis/immunology