Exp Mol Med.  2000 Jun;32(2):67-71.

Major house dust mite allergen, Der p I, activates phospholipase D in human peripheral blood mononuclear cells from allergic patients: involvement of protein kinase C

• Oh JW
• Kim JH
• Lee KS
• Han JS

Abstract

The major house-dust-mite allergen, Der p I, stimulates the phospholipase D (PLD) in peripheral blood mononuclear cells (PBMC) from allergic patients with maximal responses after 30 min exposure. At 30 min, Der p I stimulated PLD activity by 1.4-fold in mild, 1.6-fold in moderate and 2-fold in severe allergic patients over control values (p < 0.05). When the cells were pretreated for 24 h with phorbol myristate acetate to down-regulate protein kinase C (PKC), PLD stimulation by Der p I was largely abolished. These results indicate that in PBMC from allergic patients, Der p I can stimulate PLD activity, and that PKC activation is involved in this stimulation.

Keyword

Der p I; phospholipase D; protein kinase C; allergy; PBMC

MeSH Terms

Adult
Allergens/metabolism*
Allergens/immunology
Animal
Down-Regulation (Physiology)
Glycoproteins/metabolism*
Glycoproteins/immunology
Human
Hypersensitivity/metabolism
Hypersensitivity/immunology
Hypersensitivity/blood
IgE/blood
In Vitro
Leukocytes, Mononuclear/metabolism
Leukocytes, Mononuclear/immunology
Mites/metabolism
Mites/immunology
Phospholipase D/metabolism*
Phospholipase D/immunology
Protein Kinase C/metabolism*
Skin Tests
Tetradecanoylphorbol Acetate/pharmacology