Exp Mol Med.  2004 Oct;36(5):486-492.

Der f 2 activates phospholipase D in human T lymphocytes from Dermatophagoides farinae specific allergic individuals: Involvement of protein kinase C-alpha

Affiliations
  • 1Department of Pediatrics, College of Medicine, Hanyang University Seoul 133-791, Korea.
  • 2Institute of Biomedical Science, Department of Biochemistry College of Medicine, Hanyang University Seoul 133-791, Korea. jshan@hanyang.ac.kr
  • 3The Research Center for Bio-Medicinal Resources, PaiChai University, Daejon 302-735, Korea.

Abstract

The major house-dust mite allergen, Der f 2, stimulates the phospholipase D (PLD) in T lymphocytes from Dermatophagoides farinae specific allergic individuals. PLD activity increased more than two-fold in T cells from allergic patients compared with those cells from normal controls with maximal responses within 30 min after exposure of Der f 2. A well-known PLD activator PKC-alpha was found to be translocated to membrane from cytosol in Der f 2-treated T cells from Dermatophagoides farinae specific allergic individuals. Down-regulation of PKC-alpha with phorbol myristate acetate pretreatment for 24 h abolished Der f 2-induced PLD activation. Ro 320432, PKC inhibitor also reduced the effects of Der f 2-induced PLD activation suggesting that PKC-alpha acts as upstream activator of PLD in Der f 2-treated T cells. Taken together, the present data suggest that Der f 2 can stimulate PLD activity through the PKC-alpha activation in T cells from Dermatophagoides farinae allergic individuals

Keyword

PKC-alpha, allergy; Der f2; phospholipase D; T lymphocytes

MeSH Terms

Adolescent
Adult
Animals
Antigens, Dermatophagoides/*immunology
Dermatophagoides farinae/*immunology
Female
Humans
Hypersensitivity, Immediate/*enzymology/*immunology
Male
Phospholipase D/*metabolism
Protein Kinase C/antagonists & inhibitors/*physiology
Research Support, Non-U.S. Gov't
Skin Tests
T-Lymphocytes/*enzymology/immunology
Tetradecanoylphorbol Acetate/*analogs & derivatives/pharmacology
Up-Regulation
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