J Vet Sci.  2008 Jun;9(2):203-205. 10.4142/jvs.2008.9.2.203.

Aquaporin 1 expression in tissues of canines possessing inherited high K+ erythrocytes

• Ochiai H
• Hishiyama
• Hisamatsu
• Kanemaki N

• KMID: 1106237
• DOI: http://doi.org/10.4142/jvs.2008.9.2.203

Abstract

We investigated the expression of aquaporin 1 (AQP1) in tissues from canines with an inherited anomaly that causes their erythrocytes to have high K+. Northern blot analysis revealed abundant AQP1 expression in lung and kidney, though little expression was found in spleen. Using anti-C-terminus for dog AQP1, abundant expression was shown in kidney, trachea, and eye, but little expression was shown in pancreas and cerebrum, indicating that AQP1 expression in canine tissues is similar to that noted in other mammals.

Keyword

aquaporin 1 expression; canine; erythrocyte

MeSH Terms

Animals
Aquaporin 1/*metabolism
Blotting, Northern
Dogs
Erythrocytes/*chemistry
Immunoblotting
Potassium/*analysis
Viscera/metabolism

Figure

• 

  Fig. 1 Northern blot analysis of AQP1 expression in dog tissues (A). Each 10 µg sample of mRNA was purified, electrophoresed, and blotted. Hybridization of this blot with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to ensure RNA integrity is also shown (B). AQP1 expression of each tissue was standardized using the signal intensity of GAPDH (C).

• 

  Fig. 2 Immunoblotting of membranes isolated from various HK and LK dog tissues. Membrane protein samples (10 µg) were electrophoresed on 12% polyacrylamide gels and immunoblotted with anti-dog AQP1 serum.

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