Korean J Parasitol.
2010 Dec;48(4):319-324. 10.3347/kjp.2010.48.4.319.
Molecular Cloning of Plasmodium vivax Calcium-Dependent Protein Kinase 4
- Affiliations
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- 1Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Korea. tongsookim@inha.ac.kr
- 2Department of Pathology, University of Florida, J-566, 1600 SW Archer Road, Gainesville, FL 32610, USA.
- 3Department of Entomology, Armed Forces Research Institute of Medical Science, Bangkok 10400, Thailand.
- 4Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751, Korea.
- 5Institute of Global Environment and Department of Biology, Kyung Hee University, Seoul 130-701, Korea.
- 6Department of Parasitology, Inha University College of Medicine, Incheon 400-712, Korea.
- KMID: 1104699
- DOI: http://doi.org/10.3347/kjp.2010.48.4.319
Abstract
- A family of calcium-dependent protein kinases (CDPKs) is a unique enzyme which plays crucial roles in intracellular calcium signaling in plants, algae, and protozoa. CDPKs of malaria parasites are known to be key regulators for stage-specific cellular responses to calcium, a widespread secondary messenger that controls the progression of the parasite. In our study, we identified a gene encoding Plasmodium vivax CDPK4 (PvCDPK4) and characterized its molecular property and cellular localization. PvCDPK4 was a typical CDPK which had well-conserved N-terminal kinase domain and C-terminal calmodulin-like structure with 4 EF hand motifs for calcium-binding. The recombinant protein of EF hand domain of PvCDPK4 was expressed in E. coli and a 34 kDa product was obtained. Immunofluorescence assay by confocal laser microscopy revealed that the protein was expressed at the mature schizont of P. vivax. The expression of PvCDPK4-EF in schizont suggests that it may participate in the proliferation or egress process in the life cycle of this parasite.
MeSH Terms
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Amino Acid Sequence
Cloning, Molecular
Conserved Sequence
Escherichia coli/genetics
Gene Expression
Gene Expression Profiling
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Sequence Data
Molecular Weight
Plasmodium vivax/chemistry/*genetics
Protein Kinases/analysis/chemistry/*genetics
Protein Structure, Tertiary
Protozoan Proteins/analysis/chemistry/*genetics
Sequence Alignment
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