J Vet Sci.  2000 Jun;1(1):49-52.

Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex

• Park BK
• Park YH
• Seo KS

Abstract

Thirty-nine healthy pigs (28-32 days old) were purchased from a commercial swine farm and housed at swine pens of the College. The animals were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (HCV, LOM strain) and then boostered at 5 weeks after the first vaccination. The animals were divided into 4 experimental groups: 0.05% (w/w) PowerFeel-supplemented diet (T-1, n = 10); 3% (w/w) SuperFeed-supplemented diet (T-2, n = 10); diluted PowerFeel solution (1 : 500, v/v) as drinking water (T-3, n=9); control (n=10). PowerFeel is an original form of ionized alkali mineral complex (IAMC) and SuperFeed is a commercial product of IAMC. The subpopulation of lymphocyte in blood was assayed by a flow cytometry and HCV-specific antibody was determined by an indirect immunofluorescence assay. In IMAC-treated groups, the proportions of subpopulation expressing MHC-class II, CD2+, CD4+, CD8+, and surface IgM+ B lymphocytes were significantly decreased at 5-weeks after the first vaccination. Significant decreases were also observed in the proportions of MHC-class II, CD2+ and CD8+ lymphocyte at 3-weeks after the booster injection. The humoral immune responses in T-1 and T-2 groups were greater than those in T-3 or control group. These results suggest that IAMC-supplemented diets may have an HCV-specific immunostimulatory effect in pigs.

Keyword

Ionized alkali mineral complex; lymphocyte sub-populations; attenuated hog cholera virus vaccine

MeSH Terms

Animal Feed
Animals
Antibodies, Monoclonal/*blood/isolation & purification
Antigens, CD2/blood
B-Lymphocytes/immunology
CD4-Positive T-Lymphocytes/immunology
CD8-Positive T-Lymphocytes/immunology
Classical Swine Fever/*immunology
Classical swine fever virus/*immunology
Dietary Supplements
Ions
Lymphocyte Subsets/*immunology
*Minerals
Swine
Vaccines, Attenuated/*administration & dosage
Viral Vaccines/*administration & dosage