Exp Mol Med.  2004 Aug;36(4):336-344.

Vascular endothelial growth factor-induced angiogenic gene therapy in patients with peripheral artery disease

Affiliations
  • 1Department of Medicine, Samsung Medical Center Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710, Korea. dkkim@smc.samsung.co.kr
  • 2Division of Vascular Surgery, Samsung Medical Center Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710, Korea.
  • 3Department of Radiology Samsung Medical Center Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710, Korea.
  • 4ViroMed Co. Ltd., Seoul 151-818, Korea.
  • 5Institute of Molecular Biology and Genetics Seoul National University, Seoul 151-742, Korea.
  • 6Research Laboratories Dong-A Pharmaceutical Co. Ltd. Yongin, Gyeonggi-do 449-905, Korea.

Abstract

This phase 1 clinical trial tested the safety of intramuscular gene transfer by using naked plasmid DNA encoding the gene for VEGF, and analyzed the potential therapeutic benefits in patients with severe peripheral arterial disease (PAD). This study was an open-labeled, dose- escalating, single-center trial on nine male patients with severe debilitating PAD who had not responded to conventional therapy. Seven had Buerger's disease and two had arteriosclerosis obliterans. Plasmid DNA (pCK) containing human VEGF165 was given by eight intramuscular injections in and around the area in need of new blood vessels. The study evaluated three escalating total doses (2, 4, and 8 mg of pCK- VEGF165), with half of each total dose given four weeks apart. The follow-up duration was nine months. The gene injections were well tolerated without significant side effects or laboratory abnormalities related to gene transfer. Three patients showed transient edema in their extremities. Ischemic pain of the affected limb was relieved or improved markedly in six of seven patients. Ischemic ulcers healed or improved in four of six patients. The mean ankle-brachial index (ABI) improved significantly. Six of nine patients showed an increase in collateral vessels around the injection sites demonstrated by digital subtraction angiography. However, there was no relationship between the degree of ABI improvement and the dose given. Mean plasma levels of VEGF did not increase significantly. In conclusion, intramuscular injections of pCK- VEGF165 can be performed safely to induce therapeutic angiogenesis in patients with severe PAD.

Keyword

angiogenesis; gene therapy; peripheral vascular disease; vascular endothelial growth factor

MeSH Terms

Adult
Aged
Angiography, Digital Subtraction
Arterial Occlusive Diseases/*therapy
Foot/pathology
*Gene Therapy
Gene Transfer Techniques
Humans
Injections, Intramuscular
Male
Middle Aged
*Neovascularization, Physiologic
Peripheral Vascular Diseases/*therapy
Research Support, Non-U.S. Gov't
Vascular Endothelial Growth Factor A/*genetics
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