Korean J Parasitol.  2011 Jun;49(2):109-114. 10.3347/kjp.2011.49.2.109.

Pro-inflammatory Cytokine Expression of Spleen Dendritic Cells in Mouse Toxoplasmosis

• Nam HW
• Ahn HJ
• Yang HJ

• KMID: 1090609
• DOI: http://doi.org/10.3347/kjp.2011.49.2.109

Abstract

Dendritic cells have been known as a member of strong innate immune cells against infectious organelles. In this study, we evaluated the cytokine expression of splenic dendritic cells in chronic mouse toxoplasmosis by tissue cyst-forming Me49 strain and demonstrated the distribution of lymphoid dendritic cells by fluorescence-activated cell sorter (FACS). Pro-inflammatory cytokines, such as IL-1alpha, IL-1beta, IL-6, and IL-10 increased rapidly at week 1 post-infection (PI) and peaked at week 3 PI. Serum IL-10 level followed the similar patterns. FACS analysis showed that the number of CD8alpha+/CD11c+ splenic dendritic cells increased at week 1 and peaked at week 3 PI. In conclusion, mouse splenic dendritic cells showed early and rapid cytokine changes and may have important protective roles in early phases of murine toxoplasmosis.

Keyword

Toxoplasma gondii; spleen; dendritic cell; cytokine; FACS; mouse

MeSH Terms

Animals
Antigens, CD11c/analysis
Antigens, CD8/analysis
Cytokines/*blood/*secretion
Dendritic Cells/chemistry/*immunology
Disease Models, Animal
Flow Cytometry
Mice
Mice, Inbred BALB C
Rodent Diseases/immunology
Spleen/*immunology
Time Factors
Toxoplasmosis, Animal/*immunology