J Korean Med Sci.  1998 Apr;13(2):165-170. 10.3346/jkms.1998.13.2.165.

Chemokines, osteopontin, ICAM-1 gene expression in cultured rat mesangial cells

• Lee SK
• Park JY
• Chung SJ
• Yang WS
• Kim SB
• Park SK
• Park JS

• KMID: 1064964
• DOI: http://doi.org/10.3346/jkms.1998.13.2.165

Abstract

To investigate whether MCP-1, CINC, RANTES, osteopontin and ICAM-1 mRNA could be induced in cultured rat mesangial cells by interleukin-1beta(IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and lipopolysaccharide (LPS), and whether MCP-1 and CINC gene expression could be modulated by dexamethasone, Northern blot assays were performed. IL-1beta induced MCP-1, CINC, RANTES and ICAM-1 gene expression in a time dependent manner. IL-1beta-induced MCP-1, CINC and ICAM-1 mRNA amount were maximal at 3 hours exposure around 14.5, 15.7, 2.2 folds increase and IL-1beta-induced RANTES mRNA at 24 hours around 2.0 folds. TNF-alpha and LPS also induced MCP-1 and ICAM-1 gene expression. TNF-alpha also induced RANTES gene expression but LPS did not. On the other hand, IL-1beta, TNF-alpha and LPS had little effect on osteopontin gene expression but fetal calf serum could increase osteopontin mRNA. Dexamethasone suppressed the IL-1beta-induced MCP-1 and CINC mRNA. These results suggest that, through these gene expressions, mesangial cells are able to communicate directly or indirectly with macrophages or neutrophils, which may lead to glomerulosclerosis.

MeSH Terms

Animal
Cells, Cultured
Chemokines/genetics*
Chemokines, CXC/genetics
Chemotactic Factors/genetics
Dexamethasone/pharmacology
Gene Expression
Glomerular Mesangium/metabolism*
Growth Substances/genetics
Intercellular Adhesion Molecule-1/genetics*
Interleukin-1/pharmacology
Monocyte Chemoattractant Protein-1/genetics
RANTES/genetics
Rats
Rats, Sprague-Dawley
Sialoglycoproteins/genetics*