Exp Mol Med.
2011 Dec;43(12):676-683. 10.3858/emm.2011.43.12.078.
Effects of ferulic acid on diabetic nephropathy in a rat model of type 2 diabetes
- Affiliations
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- 1Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju 220-701, Korea. cchung@yonsei.ac.kr
- 2Department of Nursing, Gachon University of Medicine and Science, Incheon 406-799, Korea.
- 3Department of Internal Medicine, Soonchunhyang University College of Medicine, Cheonan 331-090, Korea.
- 4Department of Anatomy, Yonsei University Wonju College of Medicine, Wonju 220-701, Korea.
- 5Institute of Lifestyle Medicine, Yonsei University Wonju College of Medicine, Wonju 220-701, Korea.
- KMID: 1058705
- DOI: http://doi.org/10.3858/emm.2011.43.12.078
Abstract
- Diabetic nephropathy is the most serious complication in diabetes mellitus. It is known that oxidative stress and inflammation play a central role in the development of diabetic nephropathy. In this study, we investigated that ferulic acid (FA) known as anti-oxidative agent could effect on diabetic nephropathy by anti-oxidative and anti-inflammatory mechanism. We examined the effects of FA in obese diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats and non-diabetic control Long-Evans Tokushima Otsuka (LETO) rats. We treated FA to experimental rats from 26 to 45 weeks of age. We evaluated ACR, MDA and MCP-1 in 24 h urine and examined renal histopathology and morphologic change in extracted kidneys from rats. Also, we evaluated the ROS production and MCP-1 levels in cultured podocyte after FA treatment. In the FA-treated OLETF rats, blood glucose was significantly decreased and serum adiponectin levels were increased. Urinary ACR was significantly reduced in FA-treated OLETF rats compared with diabetic OLETF rats. In renal histopathology, FA-treated OLETF rats showed decreased glomerular basement membrane thickness, glomerular volume, and mesangial matrix expansion. FA treatment decreased oxidative stress markers and MCP-1 levels in 24 h urine of rats and supernatants of cultured podocyte. In conclusion, it was suggested that FA have protective and therapeutic effects on diabetic nephropathy by reducing oxidative stress and inflammation.
Keyword
MeSH Terms
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Animals
Anti-Inflammatory Agents, Non-Steroidal/*therapeutic use
Antioxidants/*therapeutic use
Cells, Cultured
Chemokine CCL2/genetics/urine
Collagen/analysis
Coumaric Acids/*therapeutic use
Diabetes Mellitus, Type 2/*complications
Diabetic Nephropathies/*complications/*drug therapy/pathology
Gene Expression/drug effects
Kidney/drug effects/pathology
Malondialdehyde/urine
Podocytes/drug effects/metabolism
Rats
Rats, Inbred OLETF
Reactive Oxygen Species/metabolism
Transforming Growth Factor beta1/analysis
