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Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column

Seo MG, Kim TH, Kwon OD, Jeong KS, Lee YJ, Kim SJ, Park SJ, Kwon YS, Kwak DM

Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar...
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Plasma Fibronectin Levels in Patients with Psoriasis

Sung KY, Chung SL

  • KMID: 2231291
  • Korean J Dermatol.
  • 1989 Oct;27(5):495-501.
Fibronectin(FN) is a physiologic glycoprotein presenton cell surface and in connective tissue, extracellular matrix and plasma. Increased plasma FN levels have been reported in the patients with psoriasis, connective tissue...
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Usefulness of Disposable Horizontal Electrophoresis

Kim KH, Oh JS, Lee HC, Chung J, Lee SE

  • KMID: 1540638
  • J Korean Soc Microbiol.
  • 1997 Oct;32(5):577-581.
We designed a new electrophoresis apparatus. This was consisted of the body into which agarose could be poured directly and the cover from which the platinium electrodes were suspended. After...
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Extra-LDH Isoenzyme(LD₆) in Myocardial Infarction with Serious Course: Two cases report

Kim KD, Choi MS, Lee CH, Jeon CH, Kim CS, Kim YJ

Serum lactate dehydrogenase isoenzyme are separated 5 fractions (LD1-LD5) in normal serum by agarose electrophoresis. An unusual band on the cathodic side of LD5 isoenzyme has been found on lactate dehydrogenase...
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Molecular cloning and expression of 3beta-hydroxy steroid dehydrogenase in E-Coli

Oh JM

  • KMID: 1937887
  • Korean J Anat.
  • 1998 Oct;31(5):771-778.
This study was carried out to obtain the 3beta-hydroxy-5-ene steroid dehydrogenase (3beta-HSD) which is contained in human trophoblasts. To produce 3beta-HSD cDNA, reverse transcription-polymerase chain reaction (RT-PCR) with the total...
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The Purification of Fish B - like Cells and Their Functional Properties

Choi SH

  • KMID: 1547850
  • Korean J Immunol.
  • 1997 Mar;19(1):113-120.
Serum immunoglobulins from carp Cyprinus carpio were purified using affinity chromatography methods. Fish were immunized with bovine serum albumin (BSA) and the specific fish antibodies purified from the immune serum...
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Detection of Antibody to Candida albicans Proteinase in Sera from C. albicans - Cultured Patients and Healthy Controls

Lee KH, Shin WS, Park HS, Uh Y, Jang IH, Yoon KJ, Koh CM

  • KMID: 1540526
  • J Korean Soc Microbiol.
  • 1997 Jun;32(3):307-314.
To investigate whether anti-Candida proteinase antibody could be a diagnostic marker, we examined seroreactivity to proteinase in sera from 90 healthy controls and 8 of C. albicans culture-positive patients. Previously...
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In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks

Kim KH, Choi SJ, Choi YI, Kim L, Park IS, Han JY, Kim JM, Chu YC

  • KMID: 2277440
  • Korean J Pathol.
  • 2013 Jun;47(3):238-244.
BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of...
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Preparation of Compact Agarose Cell Blocks from the Residues of Liquid-Based Cytology Samples

Choi SJ, Choi YI, Kim L, Park IS, Han JY, Kim JM, Chu YC

BACKGROUND: Inevitable loss of diagnostic material should be minimized during cell block preparation. We introduce a modified agarose cell block technique that enables the synthesis of compact cell blocks by...
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The Study of the Preparation of Purified Fibronectin from the Human Plasma

Kim JC, Kim IS, Kim HC, Shyn KH, Koo BS, Lee HS

  • KMID: 1949238
  • J Korean Ophthalmol Soc.
  • 1989 Aug;30(4):189-197.
Fibronectin(FN), a glycoprotein present in plasma and extracellular matrix, has been reported to be effective on various corneal disorders such as persistent epithelial defect, corneal trophic ulcer, herpetic keratitis, etc....
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Studies on the protein biosynthesis in isolated periodontal ligament

Chung HI

  • KMID: 2271422
  • Korean J Orthod.
  • 1979 Dec;9(1):9-14.
The purpose of this study was to pursue the biosynthesis of proteins of human and bovine periodontal ligaments in vitro system. The excised periodontal ligaments from human and bovine were...
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Factor V Gene Polymorphism with Preeclamptic Women in Korea

Hong SH, Kim JK, Choi JW, Ji IW, Jeong EH, Kim HS

  • KMID: 2076730
  • Korean J Obstet Gynecol.
  • 2002 Nov;45(11):1912-1916.
OBJECTIVE: This study was to evaluate the association of the G1691A mutation of factor V gene with preeclamptic patients in Korea. METHODS: Factor V genotype were determined in 113 preeclamptic patients...
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Serum protein concentrations and electrophoretic patterns by categories of small opacity profusions in coal workers' pneumoconioses

Hong JP, Chung HK

  • KMID: 2282494
  • Korean J Prev Med.
  • 1991 Mar;24(1):1-7.
The concentrations of serum proteins fractions and their electrophoretical patterns were investigated in 135 patients with coal workers' pneumoconiosis who participated in confirmative examination for pneumoconiosis in December 1989. Their...
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A Case of Hyperlipoproteinemia Type II b with Diabetes Mellitus

Kim TJ, Chyung EJ, Park SY, Yang SK, Kim JT

  • KMID: 1678640
  • Korean J Dermatol.
  • 1982 Dec;20(6):985-989.
A 32-year-old male patient with uncontrolled diabetes mellitus was affected with hyperlipoproteinemia type II His skin lesion showed yellowish papulsr tuberoruptive xanthoma on forearm, shoulder, elbow and knee. Besides skin...
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The Effect of Hyaluronic Acid and Epidermal Growth Factor on Cultured Corneal Epithelial Cells in the Rabbit

Lee EK, Jin KH, Kim JM

  • KMID: 2123178
  • J Korean Ophthalmol Soc.
  • 1993 Oct;34(10):978-984.
Corneal epithelial wound healing is followed by cellular migration and proliferation. Hyaluronic acid and EGF have been used to promote corneal wound healing. We investigated the effect and action mechanism...
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The Binding Properties of Glycosylated and Non-Glycosylated Tim-3 Molecules on CD4+CD25+ T Cells

Lee MJ, Heo YM, Hong SH, Kim K, Park S

BACKGROUND: T cell immunoglobulin and mucin domain containing 3 protein (Tim-3) expressed on terminally differentiated Th1 cells plays a suppressive role in Th1-mediated immune responses. Recently, it has been shown...
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Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification

Lee MK, Kim HR

BACKGROUND: Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and...
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Mucin Secretion and MUC1 mRNA Expression in the Cultured Secretory Cells of the Rat Nasal Epithelium

Kim IT

  • KMID: 2223260
  • J Rhinol.
  • 1999 May;6(1):30-36.
The upper respiratory system is lined with epithelium (mucosa) of the pseudostratified ciliated columnar type that produces mucus as a secretion. The major constituent of mucus is mucin, a glycoprotein...
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Expression and Purification of the Capsid Protein of the Japanese Encephalitis Virus and Production of its Polyclonal Antibody

Park JS, Kim JM, Yun SI, Choi YJ, Song BH, Yeon SM, Kim SY, Hahn YS, Park HJ, Kim MJ, Lee YM

  • KMID: 2168481
  • J Bacteriol Virol.
  • 2005 Mar;35(1):49-55.
Japanese encephalitis virus (JEV) is a member of Flaviviruses, transmitted by mosquitoes. The core of JEV is composed of the capsid (C) proteins. In order to produce the recombinant viral...
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Production and Assessment of Goat Antihuman Globulin Reagent

Chung JL, Yong DE, Kim MJ, Kim HO, Cho YS

  • KMID: 2300153
  • Korean J Blood Transfus.
  • 1997 Dec;8(2):177-185.
BACKGROUND: Since the 1960 s rabbit antihuman globulin reagent has been used widely. Recently most conjugate of enzyme immunoassay is produced from goat, and precise purification method is developed. Therefore...
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