J Korean Ophthalmol Soc.  1993 Oct;34(10):978-984.

The Effect of Hyaluronic Acid and Epidermal Growth Factor on Cultured Corneal Epithelial Cells in the Rabbit

• Lee EK
• Jin KH
• Kim JM

Abstract

Corneal epithelial wound healing is followed by cellular migration and proliferation. Hyaluronic acid and EGF have been used to promote corneal wound healing. We investigated the effect and action mechanism of Hyaluronic acid and EGF using agarose explant method and organ culture. In the agarose explant method, we studied the promoting effect of different concentration of Hyaluronic acid and EGF, combined effects and mitotic inhibitory effect of colchicine on cultured corneal epithelial cells in the rabbit. And we studied the result of the organ culture with same agents using round epithelial defect by the trephine. Hyaluronic acid and EGF showed significant promoting effect on corneal wound healing, and the combined effect was also significant. The effect of Hyaluronic acid and EGF was inhibited in colchicine combined culture. Therefore, the effect of hyaluronic acid and EGF is seemed to be on the cell proliferation rather than cell motility.

Keyword

Colchicine; Cornea; Culture; Epidermal Growth Factor; Hyaluronic acid

MeSH Terms

Cell Movement
Cell Proliferation
Colchicine
Cornea
Epidermal Growth Factor*
Epithelial Cells*
Hyaluronic Acid*
Organ Culture Techniques
Sepharose
Wound Healing
Colchicine
Epidermal Growth Factor
Hyaluronic Acid
Sepharose