- Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells
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Gao J, Meng C, Chen Z, Li C, Liu G
- KMID: 1712311
- J Vet Sci.
- 2013 Dec;14(4):441-447.
- doi: 10.4142/jvs.2013.14.4.441
Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit... -
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- A role of carboxy-terminal region of Toxoplasma gondii-heat shock protein 70 in enhancement of T. gondii infection in mice
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Mun HS, Norose K, Aosai F, Chen M, Yano A
- KMID: 1715245
- Korean J Parasitol.
- 2000 Jun;38(2):107-110.
- doi: 10.3347/kjp.2000.38.2.107
We investigated the role of recombinant Toxoplasma gondii heat shock protein (rT.g.HSP) 70-full length, rT.g.HSP70-NH2-terminal region, or rT.g.HSP70-carboxy-terminal region in prophylactic immunity in C57BL/6 mice perorally infected with Fukaya cysts... -
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- Purification and characterization of recombinant murine endostatin in E. coli
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You WK, So SH, Lee H, Park SY, Yoon MR, Chang SI, Kim HK, Joe YA, Hong YK, Chung SI
- KMID: 1029927
- Exp Mol Med.
- 1999 Dec;31(4):197-202.
Endostatin, a carboxyl-terminal fragment of collagen XVIII is known as an anti-angiogenic agent, that specifically inhibits the proliferation of endothelial cell and the growth of several primary tumor.... -
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- Expression of a recombinant branched chain alpha-oxo acid dehydrogenase complex E2 (BCOADC-E2) in insect cells and its immunoreactivity to autoimmune sera
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Lee SM, Ko SH, Park JE, Cha SH
- KMID: 754990
- Exp Mol Med.
- 1998 Jun;30(2):65-71.
Preparation of a pure autoantigen by way of recombinant DNA technology has an important value in an accurate diagnosis or prognosis of an autoimmune disease. BCOADC-E2 subunit, a mitochondrial protein, has been... -
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- Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice
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Yang DK, Kweon CH, Kim BH, Lim SI, Kwon JH, Kim SH, Song JY, Han HR
- KMID: 1114853
- J Vet Sci.
- 2005 Jun;6(2):125-133.
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of... -
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- Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus
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Lee WJ, Shin MK, Cha SB, Yoo HS
- KMID: 1712314
- J Vet Sci.
- 2013 Dec;14(4):467-472.
- doi: 10.4142/jvs.2013.14.4.467
Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This virus is an important zoonotic agent and a public concern worldwide. Infected pigs are... -
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- Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
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Reyes AW, Simborio H, Hop HT, Arayan LT, Kim S
- KMID: 2363344
- J Vet Sci.
- 2016 Mar;17(1):119-122.
- doi: 10.4142/jvs.2016.17.1.119
The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but... -
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- Synthesis of recombinant blood coagulation factor VIII (FVIII) heavy and light chains and reconstitution of active form of FVIII
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Oh SH, Lee MY, Song DW
- KMID: 1029938
- Exp Mol Med.
- 1999 Jun;31(2):95-100.
FVIII is synthesized as a single chain precursor of approximately 280 kD with the domain structure of A1-A2-B-A3-C1-C2 and it circulates as a series of metal ion-linked heterodimers that result from cleavages... -
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- Characterization and localization of the unique Marek's disease virus type 2 ORF873 gene product
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Jang HK
- KMID: 1099833
- J Vet Sci.
- 2004 Sep;5(3):207-213.
Studies on Marek's disease virus (MDV)-unique genes are important for understanding the biological nature of the virus. Based on complete DNA sequence analyses of the MDV genomes, the MDV genomes... -
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- Membrane transducing activity of recombinant Hoxc8 protein and its possible application as a gene delivery vector
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Kim ES, Kang M, Park HW, Kim MH
- KMID: 1052937
- Exp Mol Med.
- 2008 Apr;40(2):151-160.
- doi: 10.3858/emm.2008.40.2.151
In order to examine whether the Hoxc8 protein can deliver nucleic acid into mammalian cells, we designed several Hoxc8-derived recombinant proteins to be synthesized as glutathione S-transferase (GST) fused forms... -
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- Biochemical properties of full-length hepatitis C virus RNA-dependent RNA polymerase expressed in insect cells
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Choi HB, Kim YG, Oh JW
- KMID: 1119328
- Exp Mol Med.
- 2003 Dec;35(6):475-485.
The hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B protein, is the key viral enzyme responsible for replication of the HCV viral RNA genome. Although several full-length and truncated forms... -
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- Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus
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Muleke CI, Ruofeng Y, Lixin X, Yanming S, Xiangrui L
- KMID: 1089909
- J Vet Sci.
- 2006 Sep;7(3):249-255.
- doi: 10.4142/jvs.2006.7.3.249
Because of the complexity of the cathepsin B-like (CBL) family, an information on the biological and biochemical characteristics of individual CBL genes is lacking. In this study, we investigated the... -
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- C/EBP binding activity to site F of the rat GLUT2 glucose transporter gene promoter is attenuated by c-Jun in vitro
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Kim JW, Ahn YH
- KMID: 755211
- Exp Mol Med.
- 2002 Nov;34(5):379-384.
The expression of the GLUT2 glucose transporter gene in liver is suppressed in cultured hepatoma cell lines and primary cultured hepatocytes. Earlier report showed that CCAAT/enhancer binding protein (C/EBP) regulates... -
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- The inhibitory effects of recombinant plasminogen kringle 1-3 on the neovascularization of rabbit cornea induced by angiogenin, bFGF, and VEGF
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Kim JH, Kim JC, Shin SH, Chang SI, Lee HS, Chung SI
- KMID: 1029928
- Exp Mol Med.
- 1999 Dec;31(4):203-209.
Angiostatin is a potent angiogenesis inhibitor that is composed of the first four kringles of plasminogen fragment. Angiostatin with one less kringle molecule (kringle 1 to 3) was... -
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- Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice
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Zhao H, Wang Y, Ma Z, Wang Y, Feng WH
- KMID: 1784640
- J Vet Sci.
- 2014 Jun;15(2):199-208.
- doi: 10.4142/jvs.2014.15.2.199
Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly,... -
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- Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
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He XY, Ma LB, He XN, Si WT, Zheng YM
- KMID: 2413163
- J Vet Sci.
- 2016 Jun;17(2):145-152.
- doi: 10.4142/jvs.2016.17.2.145
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target... -
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- Interferon-gamma upregulates the stromelysin-1 gene expression by human skin fibroblasts in culture
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Lee KS, Ryoo YW, Song JY
- KMID: 754989
- Exp Mol Med.
- 1998 Jun;30(2):59-64.
The equilibrium between deposition and degradation of extracellular matrix(ECM) is essential to normal tissue development and repair of wound or inflammatory responses. It has recently become apparent that several cytokines and growth factors... -
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- Removal of Alpha-Gal Epitopes from Porcine Aortic Valve and Pericardium using Recombinant Human Alpha Galactosidase A
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Park S, Kim WH, Choi SY, Kim YJ
- KMID: 1783143
- J Korean Med Sci.
- 2009 Dec;24(6):1126-1131.
- doi: 10.3346/jkms.2009.24.6.1126
It has been reported that the immune response due to alpha-Gal epitopes is an important factor in tissue valve failure. The elimination of the interaction between the natural anti-Gal antibodies... -
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- Characterization of yeast deoxyhypusine synthase: PKC-dependent phosphorylation in vitro and functional domain identification
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Kang KR, Chung SI
- KMID: 1029929
- Exp Mol Med.
- 1999 Dec;31(4):210-216.
The biosynthesis of hypusine [Nepsilon-(4-amino-2-hydroxybutyl)-lysine] occurs in the eIF-5A precursor protein through two step posttranslational modification involving deoxyhypusine synthase which catalyzes transfer of the butylamine moiety of... -
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- Effects of Val34Leu and Val35Leu polymorphism on the enzyme activity of the coagulation factor XIII-A
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Lee IH, Chung SI, Lee SY
- KMID: 755212
- Exp Mol Med.
- 2002 Nov;34(5):385-390.
Change in fibrin stabilizing activity of factor XIII A subunit (FXIII-A) caused by a specific mutation, Val34Leu, is recently implicated to incidences of pathophysiology of thrombosis. In an effort to... -
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