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Tactics of Mycobacterium avium subsp. paratuberculosis for intracellular survival in mononuclear phagocytes

Woo SR, Czuprynski CJ

Johne's disease is a condition that refers to chronic granulomatous enteritis in ruminants. It is believed that survival and replication of Mycobacterium (M.) paratuberculosis in mononuclear phagocytes plays an important...
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Development of vaccines to Mycobacterium avium subsp. paratuberculosis infection

Park HT, Yoo HS

Johne's disease or paratuberculosis is a chronic debilitating disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). The disease causes significant economic losses in livestock industries worldwide. There are...
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Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces

Park HT, Shin MK, Sung KY, Park HE, Cho YI, Yoo HS

  • KMID: 1700792
  • Korean J Vet Res.
  • 2014 Mar;54(1):55-57.
Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) has extended latent periods of infection. Due to this property, difficulties in the detection of fecal shedder have been raised. A newly...
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Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick

Zhao G, Wang H, Hou P, He C, He H

Paratuberculosis (Johne's disease) is a chronic debilitating disease of domestic and wild ruminants. However, widespread point-of-care testing is infrequent due to the lack of a robust method. The isothermal recombinase...
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Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine

Lee J, Moon C, Kim J, Jung C, Lee KH, Joo HG, Ahn M, Shin T

The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the...
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Genetic diversity of bovine Mycobacterium avium subsp. paratuberculosis discriminated by IS1311 PCR-REA, MIRU-VNTR, and MLSSR genotyping

Park HT, Park HE, Park WB, Kim S, Hur TY, Jung YH, Yoo HS

The aim of this study was to describe the genetic diversity of Mycobacterium avium subsp. paratuberculosis (MAP) obtained from individual cows in Korea. Twelve MAP-positive fecal DNA samples and 19...
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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis

Salgado M, Verdugo C, Heuer C, Castillo P, Zamorano P

PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting...
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A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples

Kumanan V, Nugen SR, Baeumner A, Chang YF

A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small...
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Discovery of antigens for early detection of Mycobacterium avium subsp. paratuberculosis and analysis of characteristics using bioinformatics tools

Park HT, Park HE, Shin MK, Cho YI, Yoo HS

Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is one of the most widespread and economically important diseases in cattle. Current diagnostic methods are based on the detection of...
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Gene Expression Profiles of Th1-type Chemokines in Whole Blood of Mycobacterium avium subsp. paratuberculosis-Infected Cattle

Shin MK, Park HE, Park HT, Jung M, Kang HL, Baik SC, Lee WK, Jung YH, Yoo HS

Johne's disease (JD) is a chronic, debilitating disease of ruminants including cows, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). MAP is not only important in animal husbandry, but...
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Potential biomarkers as an indicator of vertical transmission of Johne's disease in a Korean native cattle farm

Park HT, Park HE, Cho YI, Kim EH, Jung M, Shin SW, Lee SH, Kim DY, Yoo HS

Paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is one of the most widespread and economically important diseases in cattle. After birth, calves are raised with natural...
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Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea

Park KT, Ahn J, Davis WC, Koo HC, Kwon NH, Jung WK, Kim JM, Hong SK, Park YH

Paratuberculosis (PTB) is a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to...
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The Role of Bacteria in the Pathogenesis of Inflammatory Bowel Disease

Friswell M, Campbell B, Rhodes J

  • KMID: 1974129
  • Gut Liver.
  • 2010 Sep;4(3):295-306.
Crohn's disease (CD) and ulcerative colitis (UC) have features that suggest bacterial involvement, and all genetic models of inflammatory bowel disease (IBD) require the presence of commensal bacteria. CD is...
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