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Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti

Kang SW, Choi EJ, Kweon CH

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned...
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Evolution of Genetic Polymorphisms of Plasmodium falciparum Merozoite Surface Protein (PfMSP) in Thailand

Kuesap J, Chaijaroenkul W, Ketprathum K, Tattiyapong P, Na-Bangchang K

Plasmodium falciparum malaria is a major public health problem in Thailand due to the emergence of multidrug resistance. The understanding of genetic diversity of malaria parasites is essential for developing...
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Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China

Zhang Y, Lv Y, Zhang F, Zhang W, Wang J, Cui Y, Wang R, Jian F, Zhang L, Ning C

Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621...
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PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples

Moon JH, Cho SH, Yu JR, Lee WJ, Cheun HI

Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E....
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Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia

Kamaruddin M, Tokoro M, Rahman MM, Arayama S, Hidayati A, Syafruddin D, Asih PB, Yoshikawa , Kawahara E

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from...
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Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea

Kim HY, Kim YA, Lee HS, Rhie HG, Cho SH, Yu JR, Lee SE

Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2...
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Expression of Exogenous Human Hepatic Nuclear Factor-1alpha by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2

Liao S, Liu Y, Zheng B, Cho PY, Song HO, Lee YS, Jung SY, Park H

The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice,...
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Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates

Kho WG, Chung JY, Sim EJ, Kim DW, Chung WC

The present study was designed to investigate polymorphism in Duffy binding protein (DBP) gene of Plasmodium vivax isolates of Korea. Thirty samples were obtained from P. vivax patients in Yonchon-gun,...
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Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea

Kim K, Yoon S, Cheun HI, Kim JH, Sim S, Yu JR

Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunistic pathogens in HIV-positive patients. However, there is increasing evidence that these organisms can also cause gastrointestinal and ocular infections...
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Internal Amplification Control for a Cryptosporidium Diagnostic PCR: Construction and Clinical Evaluation

Hawash Y, Ghonaim MM, Al-Hazmi A

Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it...
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Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA

Rahman MM, Yagita K, Kobayashi , Oikawa Y, Hussein AI, Matsumura , Tokoro M

Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by...
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Molecular Phylogeny of Acanthamoeba

Kong HH

After morphological grouping of Acanthamoeba by Pussard and Pons, phylogeny of the genus has been always a big topic to the researchers. Because of the variability of morphological characteristics, unchangeable...
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Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene

Yu HS, Hwang MY, Kim TO, Kim TH, Kong HH, Chung DI, Park JW

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close inter-relationships. Twenty-five isolates representing 20 species were...
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Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates

Xuan YH, Chung BS, Hong YC, Kong HH, Hahn TW, Chung DI

Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a...
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Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence

Yong TS, Lee JH, Sim S, Lee J, Min DY, Chai JY, Eom KS, Sohn WM, Lee SH, Rim HJ

Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult...
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Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis

Quan JH, Kim TY, Choi IU, Lee YH

Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the...
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Identification of newly isolated Babesia parasites from cattle in Korea by using the Bo-RBC-SCID mice

Cho SH, Kim TS, Lee HW, Tsuji M, Ishihara C, Kim JT, Wee SH, Lee CG

Attempts were made to isolate and identify Korean bovine Babesia parasite. Blood samples were collected from Holstein cows in Korea, and Babesia parasites were propagated in SCID mice with circulating...
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Susceptibility of Mice to Trypanosoma evansi Treated with Human Plasma Containing Different Concentrations of Apolipoprotein L-1

Da Silva A, Fanfa VR, Otto MA, Gressler L, Tavares KC, Lazzarotto CR, Tonin AA, Miletti LC, Duarte MM, Monteiro S

The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a...
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An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting

Hawash Y, Ghonaim MM, Al-Shehri

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round...
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Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR

Sultan DM, Khalil MM, Abdouh AS, Doleh WF, Al Muthanna AAM

Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both...
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