Korean J Parasitol.  2008 Jun;46(2):105-108. 10.3347/kjp.2008.46.2.105.

Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis

• Quan JH
• Kim TY
• Choi IU
• Lee YH

• KMID: 1089472
• DOI: http://doi.org/10.3347/kjp.2008.46.2.105

Abstract

Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the present study, genotyping of the KI-1 was performed by multilocus PCR-RFLP and microsatellite sequencing. For 9 genetic markers (c22-8, c29-2, L358, PK1, SAG2, SAG3, GRA6, BTUB, and Apico), the KI-1 and RH strains exhibited typical PCR-RFLP patterns identical to the type I strains. DNA sequencing of tandem repeats in 5 microsatellite markers (B17, B18, TUB2, W35, and TgM-A) of the KI-1 also revealed patterns characteristic of the type I. These results provide strong genetic evidence that KI-1 is a type I lineage of T. gondii.

Keyword

Toxoplasma gondii; Korean isolate; genotype

MeSH Terms

Animals
Base Sequence
Cell Line
DNA, Protozoan/genetics
Genotype
Korea
Mice
*Microsatellite Repeats
Molecular Sequence Data
Polymerase Chain Reaction
*Polymorphism, Restriction Fragment Length
Sequence Alignment
Toxoplasma/classification/*genetics/*isolation & purification