Korean J Parasitol.  2001 Jun;39(2):143-150. 10.3347/kjp.2001.39.2.143.

Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates

• Kho WG
• Chung JY
• Sim EJ
• Kim DW
• Chung WC

• KMID: 1039036
• DOI: http://doi.org/10.3347/kjp.2001.39.2.143

Abstract

The present study was designed to investigate polymorphism in Duffy binding protein (DBP) gene of Plasmodium vivax isolates of Korea. Thirty samples were obtained from P. vivax patients in Yonchon-gun, Kyonggi-do in 1998. The PCR products of the samples were subjected to sequencing and hybridization analyses of the regions II and IV of P. vivax DBP gene. Two genotypes, SK-1 and SK-2, were identified on the basis of amino acid substitution and deletion. The genotype of 10 isolates was SK-1 and that of 20 isolates was SK-2. Most of the predicted amino acids in the region II of DBP gene were conserved between the Korean isolates and Belem strain except for 4-5 amino acid substitutions. In the region IV of DBP, a 6-bp insert that was shown in the Sal-1 allele type was found in SK-1, and a 27-bp insert that was shown in the Papua New Guinea allele type was found in SK-2. In conclusion, the present findings suggest that two genotypes of P. vivax coexist in the endemic area of Korea.

MeSH Terms

Amino Acid Sequence
Animals
*Antigens, Protozoan
Base Sequence
Carrier Proteins/*analysis/chemistry/*genetics
DNA, Protozoan/genetics
Genotype
Human
Korea
Malaria, Vivax/parasitology
Molecular Sequence Data
Plasmodium vivax/*genetics/isolation & purification
Polymerase Chain Reaction
*Polymorphism (Genetics)
*Protozoan Proteins
Receptors, Cell Surface/*analysis/chemistry/*genetics
Support, Non-U.S. Gov't