- Purification of Opioid Receptor in the Presence of Sodium Ion
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Kil HY
- KMID: 1934791
- Korean J Anesthesiol.
- 1996 Jun;30(6):635-641.
- doi: 10.4097/kjae.1996.30.6.635
BACKGROUND: Purification of opioid receptor is mandatory to improve opiate analgesic medication. Recently, it was reported that sodium ion increased the number of opioid binding sites for opioid antagonist. The... -
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- Mass Spectrometry Analysis for Nitration of Proteins
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Kim KP
- KMID: 2168241
- Hanyang Med Rev.
- 2013 May;33(2):110-117.
- doi: 10.7599/hmr.2013.33.2.110
Various proteomics and immunological methods including mass spectrometry combined with both liquid and 2-D PAGE, and immunodetection have been employed to identify and characterize nitrated proteins from pathological samples. Nitrosative... -
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- Identification of a Major Cytoplasmic Laminin Binding Protein as the Heat Shock Cognate Protein 70 in Highly Metastatic Human Colon Carcinoma Cells
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Woo HJ
- KMID: 2083018
- Korean J Immunol.
- 1998 Mar;20(1):75-80.
"It is not clear yet how the laminin is made in cytoplasm and is secreted by cells, though the significance of the interaction between laminin and cells during the development,... -
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- The Study of the Preparation of Purified Fibronectin from the Human Plasma
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Kim JC, Kim IS, Kim HC, Shyn KH, Koo BS, Lee HS
- KMID: 1949238
- J Korean Ophthalmol Soc.
- 1989 Aug;30(4):189-197.
Fibronectin(FN), a glycoprotein present in plasma and extracellular matrix, has been reported to be effective on various corneal disorders such as persistent epithelial defect, corneal trophic ulcer, herpetic keratitis, etc.... -
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- The Structural Properties of Israeli Carp ( Cyprinus carpio L. ) Immunoglobulin
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Choi SH, Woo JK, Jang YS, Oh CH
- KMID: 1550800
- Korean J Immunol.
- 1999 Mar;21(1):85-92.
Serum immunoglobulins from Israeli carp (I. carp) were purified using affinity chromatography. Fish were immunized with purified mouse IgG, and the specific fish antibodies purified from the immune serum on... -
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- Characterization of the partially purified proteinase from Trichomonasvaginalis
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Min DY, Ryu JS, Hyun KH
- KMID: 1715365
- Korean J Parasitol.
- 1996 Mar;34(1):49-57.
- doi: 10.3347/kjp.1996.34.1.49
Characterization of a purified proteinase from Trichomonas vaginalis was carried out using bacitracin-sepharose affinity chromatography. Trichomonas vaginalis KT-9 isolate was used as a source of enzyme study. Proteinase activity was... -
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- Plasma Fibronectin Levels in Patients with Psoriasis
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Sung KY, Chung SL
- KMID: 2231291
- Korean J Dermatol.
- 1989 Oct;27(5):495-501.
Fibronectin(FN) is a physiologic glycoprotein presenton cell surface and in connective tissue, extracellular matrix and plasma. Increased plasma FN levels have been reported in the patients with psoriasis, connective tissue... -
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- Childbearing women of twenty and under are at greater risk than those of twenty-five and over for compromised folate status
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Kim HA, Choi JH, Lim HS
- KMID: 2313323
- Nutr Res Pract.
- 2007 Dec;1(4):254-259.
This study assessed folate intakes, folate concentrations in plasma and erythrocytes, plasma total homocysteine (tHcy) concentration, and urinary excretion of folate metabolites in Korean women with childbearing potential. A total... -
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- Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)
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Shin G, Lee H, Palaksha K, Kim Y, Lee E, Shin Y, Lee E, Park K, Jung T
- KMID: 1089916
- J Vet Sci.
- 2006 Sep;7(3):293-295.
- doi: 10.4142/jvs.2006.7.3.293
The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG... -
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- Application of Hemin-Agarose Affinity Chromatography to Enrich Proteome Components of Helicobacter pylori Strain 26695
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Kang HL, Baik SC
- KMID: 1574111
- J Bacteriol Virol.
- 2005 Jun;35(2):77-85.
The whole cell extract of Helicobacter pylori strain 26695 was treated with the hemin-agarose resin and the bound fraction was analyzed by 2-Dimensional electrophoresis. The 2-D-PAGE-displayed spots were eluted and... -
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- Molecular cloning and expression of 3beta-hydroxy steroid dehydrogenase in E-Coli
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Oh JM
- KMID: 1937887
- Korean J Anat.
- 1998 Oct;31(5):771-778.
This study was carried out to obtain the 3beta-hydroxy-5-ene steroid dehydrogenase (3beta-HSD) which is contained in human trophoblasts. To produce 3beta-HSD cDNA, reverse transcription-polymerase chain reaction (RT-PCR) with the total... -
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- Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography
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Cho MJ, Chang WH, Choi MS, Kim IS, Kang JS, Park KH, Kim HK, Cha CY, Chung HK, Rhee KH
- KMID: 1421247
- J Korean Med Sci.
- 1987 Mar;2(1):65-70.
- doi: 10.3346/jkms.1987.2.1.65
Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application... -
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- Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
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El Hakim A, Shahein YE, Abouelella AM, Selim ME
- KMID: 1089670
- J Vet Sci.
- 2007 Jun;8(2):175-180.
- doi: 10.4142/jvs.2007.8.2.175
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated... -
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- Production of CD44v6 Antibody Fragments and Comparision of Their Speciticities
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Han I, Zeon SK, Park KK
- KMID: 1550740
- Korean J Immunol.
- 1999 Dec;21(4):303-309.
CD44v6 was known as tumor marker for tumor progression and metastasis in various kinds of carcinomas. The CD44v6 monoclonal antibody was produced by cell cultures or mouse ascite fluids using... -
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- The Purification of Fish B - like Cells and Their Functional Properties
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Choi SH
- KMID: 1547850
- Korean J Immunol.
- 1997 Mar;19(1):113-120.
Serum immunoglobulins from carp Cyprinus carpio were purified using affinity chromatography methods. Fish were immunized with bovine serum albumin (BSA) and the specific fish antibodies purified from the immune serum... -
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- Production of Monoclonal Antibody Against Human 14 - 3 - 3 Zeta Isoform Expressed in Escherichia coli
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Lim BU, Kang JS, Kim JW, Choi NJ
- KMID: 2083005
- Korean J Immunol.
- 1997 Sep;19(3):391-398.
14-3-3 proteins are cytoplasmic proteins of about 29 kDa and have a minimum of seven isoforms. This protein is important in signal transduction with the ability of binding with phosphoserine... -
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- Enzymatic N-glycan analysis of 31 kDa molecule in plerocercoid of Spirometra mansoni (sparganum) and its antigenicity after chemical oxidation
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Chung YB, Kong Y, Yang HJ
- KMID: 1051419
- Korean J Parasitol.
- 2004 Jun;42(2):57-60.
- doi: 10.3347/kjp.2004.42.2.57
A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein... -
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- Autologous Immunoglobulin Therapy in Patients With Severe Recalcitrant Atopic Dermatitis: A Preliminary Report
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Nahm DH, Cho SM, Kim ME, Kim YJ, Jeon SY
- KMID: 2166949
- Allergy Asthma Immunol Res.
- 2014 Jan;6(1):89-94.
- doi: 10.4168/aair.2014.6.1.89
The management of severe recalcitrant atopic dermatitis (AD) is a challenging issue for clinicians and patients. We hypothesized that repeated intramuscular injections of autologous immunoglobulin (autologous immunoglobulin therapy: AIGT) might... -
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- Identification of Cross-linked 46 KDa Protein in Experimentally Induced Silicotic Nodule in Rat Lung
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Kim YM, Kim YJ, Lee SY
- KMID: 2075001
- Korean J Occup Environ Med.
- 2003 Jun;15(2):181-187.
OBJECTIVES: This study was conducted in order to understand the cellular events associated with silica-induced pathogenesis of the rat lung. METHODS: Silicosis was induced by an intratracheal instillation of 50 mg... -
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- Human coagulation factor VIII domain-specific recombinant polypeptide expression
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Choi SJ, Jang KJ, Lim JA, Kim HS
- KMID: 2172774
- Blood Res.
- 2015 Jun;50(2):103-108.
- doi: 10.5045/br.2015.50.2.103
BACKGROUND: Hemophilia A is caused by heterogeneous mutations in F8. Coagulation factor VIII (FVIII), the product of F8, is composed of multiple domains designated A1-A2-B-A3-C1-C2. FVIII is known to interact... -
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