Korean J Ophthalmol.  2011 Feb;25(1):42-47. 10.3341/kjo.2011.25.1.42.

Gene Expression Changes in a Rat Model of Oxygen-Induced Retinopathy

• Lee NE
• Park YJ
• Chung IY
• Seo SW
• Park JM
• Yoo JM
• Song JK

• KMID: 994411
• DOI: http://doi.org/10.3341/kjo.2011.25.1.42

Abstract

PURPOSE
To identify altered patterns of retinal mRNA expression in a rat model of oxygen-induced retinopathy (OIR).
METHODS
Sprague-Dawley rats from P2 to P14 were exposed to hyperoxia (80% oxygen) to induce OIR and then returned to normoxic conditions. Control rats were sustained in room air. Retinal gene expression between the rats of OIR and the controls was compared using cDNA microarray analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.
RESULTS
Among a total of 12,731 cDNAs analyzed by mircroarray, 13 genes were strongly up- or down-regulated (>2-fold change over controls) in the OIR rats. We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon alpha-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, beta5; fibrillin-1; spectrin beta2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.
CONCLUSIONS
We confirmed changes in expressions of various retinal genes in a rat model of OIR by microarray and RT-PCR. This study should contribute to the understanding of genetic indicators associeated with OIR.

Keyword

Oxygen-induced retinopathy; Retinal gene expression

MeSH Terms

Animals
Animals, Newborn
Down-Regulation
Gene Expression
Microarray Analysis
*Oxygen
RNA, Messenger/*metabolism
Rats
Rats, Sprague-Dawley
Retina/*metabolism
Retinal Diseases/*chemically induced/*metabolism
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation

Figure

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  Fig. 1 Diagram of the proportions of genes up- and down-regulated by 1.5-fold in oxygen-induced retinopathy rats versus controls according to function.

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  Fig. 2 Up-regulated genes of oxygen-induced retinopathy (OIR) rat retina in reverse transcriptase polymerase chain reaction. CTL = control group; P = postnatal.

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  Fig. 3 Down-regulated genes of oxygen-induced retinopathy (OIR) rat retina in reverse transcriptase polymerase chain reaction. CTL = control group; P = postnatal.

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