Exp Mol Med.  2005 Apr;37(2):111-120.

Antitumor activity of spinasterol isolated from Pueraria roots

Affiliations
  • 1Department of Bioscience and Biotechnology/Institute of Bioscience, Sejong University, Seoul, Korea. umsj@sejong.ac.kr
  • 2Chebigen Inc., 305-B, Chungmugwan, Sejong University, Seoul, Korea.
  • 3Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
  • 4Seohae Environment Science Institute, Chonbuk National University Total Research Complex, Jeonbuk, Korea.

Abstract

We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria lobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA-MB-231) in a dose- and time-dependent manner, as well as the growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17beta-estradiol, which activates both estrogen receptor a (ER alpha) and ER beta. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root.

Keyword

breast neoplasms; estrogen replacement; phytoestrogens; pueraria; receptors; estrogen; spinasterol; therapy
Full Text Links
  • EMM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2021 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr