Nat Prod Sci.  2017 Jun;23(2):132-138. 10.20307/nps.2017.23.2.132.

Synergetic Hepatoprotective Effects of Korean Red Ginseng and Pueraria Radix on the Liver Damaged-Induced by Carbon Tetrachloride (CClâ‚„) in Mice

• Hwang SH
• Wang Z
• Kang IJ
• Lim SS

• KMID: 2387064
• DOI: http://doi.org/10.20307/nps.2017.23.2.132

Abstract

This study was designed to investigate the synergetic hepatoprotective effects from a mixture of Korean Red Ginseng and Pueraria Radix on carbon tetrachloride (CClâ‚„)-induced hepatotoxicity in mice. Liver toxicity was induced by intraperitoneal administration of CClâ‚„ (0.6 mg/kg) in 12 groups of ICR mice. The negative control group was given CClâ‚„ without test samples and the normal group was given no treatment. Among treatment groups, the RGAP treatment (Korean Red ginseng acetic acid extract : Pueraria Radix water extract, w/w, 38.4:57.6) decreased CClâ‚„-elevated ALT (101.60 IU/L), AST (833.89 IU/L), and LDH (365.02 IU/L) levels in the serum, and increased the SOD (11.03 unit/mg protein) and CAT (0.37 unit/mg protein) levels and the LPO levels (59.09 µM/g tissue) more than that in the mice group with CClâ‚„-induced control group hepatotoxicity. These results suggest that administration of a mixture of Korean Red ginseng and Pueraria Radix decreases CClâ‚„-induced liver damage and enhances antioxidant activity in mice and imply that administration of the mixture in a certain ratio is more effective than single administration of either Korean Red ginseng or Pueraria Radix alone.

Keyword

Carbon tetrachloride; Red Ginseng; Pueraria Radix; Hepatoprotective; Synergetic

MeSH Terms

Acetic Acid
Animals
Carbon Tetrachloride*
Carbon*
Cats
Liver*
Mice*
Mice, Inbred ICR
Panax*
Pueraria*
Water
Acetic Acid
Carbon
Carbon Tetrachloride
Water

Figure

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  Fig. 1. Body weight change after 10-day oral administration of Red ginseng and Pueraria radix extract 1) Refer to Table 1. 2) Data are expressed as the mean ± SE (n = 7)

• 

  Fig. 2. Liver weight per 100 g of body weight, to examine the effect of CCl4 and administration of extract of Red ginseng and Pueraria radix on the weight of the organ 1) Refer to Table 1. 2) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.

• 

  Fig. 3. Effect of Red ginseng and Pueraria radix on the activity of serum alanine transaminase (a; ALT), aspartate transaminase (b; AST), and lactate dehydrogenase (c; LDH) in CCl4-treated ICR mice 1) Refer to Table 1 2) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.

• 

  Fig. 4. Effect of Red ginseng and Pueraria radix on the activity of superoxide dismutase (a; SOD), catalase (b; CAT), and lipid peroxidase (c; LPO) in CCl4-treated ICR mice 1) Refer to Table 1 2) Data are expressed as the mean ± SE (n = 7) Statistical significance of differences was calculated between C group and experiment groups. ∗P <0.05, ∗∗P <0.01, ∗∗∗P <0.001.

• 

  Fig. 5. Histopathological observation of liver tissues (H&E, × 200)

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