Exp Mol Med.  1998 Mar;30(1):41-45.

Production and characterization of monoclonal antibodies to oxidized LDL

• Choi KH
• Lee HS
• Chung HK

Abstract

Oxidized low density lipoprotein (LDL) seems to take a part in atherogenesis through direct interactions with macrophages, endothelial cells, and smooth muscle cells, and is thought to participate in renal glomerular injury. For the purpose of illustrating the role of oxidized LDL in the human diseases, monoclonal antibodies were developed and characterized, recognizing oxidized LDL-specific epitopes that do not exist on native LDL. LDL was oxidized by the incubation with CuSO4, and used as immunogen. Splenocytes from the immunized mouse and mouse myeloma cells were fused to produce hybridomas, which were screened for the secretion of oxidized LDL-specific antibodies. Immunoblot analysis and binding affinity assay showed that these monoclonal antibodies recognize malondialdehyde-conjugated peptide epitopes.

Keyword

oxidized LDL; monoclonal antibodies; atherosclerosis; renal glomerular injury

MeSH Terms

Antibodies, Monoclonal
Antibody Affinity
Antibody Specificity
Enzyme-Linked Immunosorbent Assay
Epitopes
Human
Lipoproteins, LDL/immunology*
Malondialdehyde/immunology
Malondialdehyde/analysis
Peptide Fragments/immunology
Thiobarbituric Acid Reactive Substances/analysis