Yonsei Med J.  1978 Jun;19(1):70-74. 10.3349/ymj.1978.19.1.70.

Modified Dextrose Starch Agar for the Preservation of Neisseria gonorrhoeae

• Chong Y
• Kim SO
• Lee SY

• KMID: 687755
• DOI: http://doi.org/10.3349/ymj.1978.19.1.70

Abstract

To seek a practical and inexpensive method to preserve gonococcal cultures, a few methods were compared. The following methods kept the cultures alive for only a short period of time: skim milk at -20 degrees C; tryptic soy broth with 15% glycerol at -20 degrees C; cystine tryptic agar at 35 degrees C. Most of the test cultures survived for more than 4 weeks in the following media: one half strength dextrose starch agar; one half strength dextrose starch agar with ferric nitrate; one half strength dextrose starch agar with antimicrobic CNV. Dextrose starch agar could be substituted by GC medium base with a slight modification. It is concluded that preservation of gonococci in one half strength dextrose starch agar with CNV, which produces less frequent contamination, is a practical method to maintain cultures for teaching and quality control.

MeSH Terms

Agar
Bacteriological Techniques
Culture Media*
Glucose
Neisseria gonorrhoeae*
Preservation, Biological/methods*
Starch