- Optimal site of throat swab for the isolation of beta-hemolytic streptococci
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Kim SJ
- KMID: 1422017
- J Korean Med Sci.
- 1993 Dec;8(6):453-457.
- doi: 10.3346/jkms.1993.8.6.453
The optimal site for the isolation of beta-hemolytic streptococci (BHS) from throat cultures was investigated in 164 healthy elementary school children. All throat cultures were streaked onto duplicate blood agar... -
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- Modified Dextrose Starch Agar for the Preservation of Neisseria gonorrhoeae
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Chong Y, Kim SO, Lee SY
- KMID: 687755
- Yonsei Med J.
- 1978 Jun;19(1):70-74.
- doi: 10.3349/ymj.1978.19.1.70
To seek a practical and inexpensive method to preserve gonococcal cultures, a few methods were compared. The following methods kept the cultures alive for only a short period of time:... -
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- An Evaluation of the Effect of one-day Blind Subculture in the Initial Detection of Salmonella typhi Positive Blood Cultures
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Chong Y, Yi KN, Lee SY
- KMID: 687770
- Yonsei Med J.
- 1979 Jun;20(1):33-38.
- doi: 10.3349/ymj.1979.20.1.33
During the 8-month period of May to December, 1978, a total of 3,529 blood cultures were taken from Yonsei Medical Center patients and the effect of blind subculture in the... -
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- A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
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Salgado M, Verdugo C, Heuer C, Castillo P, Zamorano P
- KMID: 1784644
- J Vet Sci.
- 2014 Jun;15(2):233-239.
- doi: 10.4142/jvs.2014.15.2.233
PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting... -
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- Evaluation of thiol broth for the culture of Salmonella typhi and other bacteria from blood
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Chong Y, Kang MS, Lee SY
- KMID: 1113856
- Yonsei Med J.
- 1990 Jun;31(2):163-167.
- doi: 10.3349/ymj.1990.31.2.163
Thiol broth is known to neutralize various antimicrobial agents. Positivity of growth of various species of bacteria from blood in thiol broth was reported as similar to that in tryptic... -
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- The Incidence and Clinical Implication of Sputum with Positive Acid-Fast Bacilli Smear But Negative in Mycobacterial Culture in a Tertiary Referral Hospital in South Korea
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Lee JS, Kim EC, Joo SI, Lee SM, Yoo CG, Kim YW, Han SK, Shim YS, Yim JJ
- KMID: 1783058
- J Korean Med Sci.
- 2008 Oct;23(5):767-771.
- doi: 10.3346/jkms.2008.23.5.767
Although it is not rare to find sputum that is positive acid-fast bacilli (AFB) smear but subsequent culture fails to isolate mycobacteria in clinical practice, the incidence and clinical implication... -
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- Development and Evaluation of the Quick Anaero-system-A New Disposable Anaerobic Culture System
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Yang NW, Kim JM, Choi GJ, Jang SJ
- KMID: 1096798
- Korean J Lab Med.
- 2010 Apr;30(2):133-137.
- doi: 10.3343/kjlm.2010.30.2.133
BACKGROUND: We developed a new disposable anaerobic culture system, namely, the Quick anaero-system, for easy culturing of obligate anaerobes. METHODS: Our system consists of 3 components: 1) new disposable anaerobic gas... -
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- A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
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Kumanan V, Nugen SR, Baeumner A, Chang YF
- KMID: 1089344
- J Vet Sci.
- 2009 Mar;10(1):35-42.
- doi: 10.4142/jvs.2009.10.1.35
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small... -
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- Comparison of Dio-Bacit, Bacitracin-Trimethoprim/ Sulphamethoxazole and Latex Agglutination in the Diagnosis of Group A Beta-Hemolytic Streptococci
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Altindis M, Aktepe OC, Kocagoz T
- KMID: 1734093
- Yonsei Med J.
- 2004 Feb;45(1):56-60.
- doi: 10.3349/ymj.2004.45.1.56
Not only is Group A beta-hemolytic Streptococcus (GAS) the most frequent cause of bacterial pharyngitis, it is also the culprit in various skin and systemic infections, acute rheumatic fever, post... -
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- The Value of Routinely Culturing for Tuberculosis During Bronchoscopies in an Intermediate Tuberculosis-Burden Country
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Kim MH, Suh GY, Chung MP, Kim H, Kwon OJ, Lee JH, Lee NY, Koh WJ
- KMID: 1786211
- Yonsei Med J.
- 2007 Dec;48(6):969-972.
- doi: 10.3349/ymj.2007.48.6.969
PURPOSE: Many medical centers routinely culture bronchoscopy samples for Mycobacterium tuberculosis, even when tuberculosis is not strongly suspected. The value of this practice, however, is controversial. We evaluated the... -
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- Growth Dynamics of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa as a Function of Time to Detection in BacT/Alert 3D Blood Culture Bottles with Various Preincubation Conditions
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Lee DH, Koh EH, Choi SR, Kim S
- KMID: 1711312
- Ann Lab Med.
- 2013 Nov;33(6):406-409.
- doi: 10.3343/alm.2013.33.6.406
BACKGROUND: Delayed entry of blood culture bottles is inevitable when microbiological laboratories do not operate for 24 hr. There are few studies reported for prestorage of these bottles. The growth... -
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- Evaluation of the Impact of Automated Specimen Inoculation, Using Previ Isola, on the Quality of and Technical Time for Stool Cultures
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Mischnik A, Trampe M, Zimmermann S
- KMID: 2363152
- Ann Lab Med.
- 2015 Jan;35(1):82-88.
- doi: 10.3343/alm.2015.35.1.82
BACKGROUND: This study was designed as a quasi-experiment to evaluate automatic inoculation of fecal specimens, using the automated specimen inoculator Previ Isola (bioMerieux, France). METHODS: We evaluated the quality of cultures,... -
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- Clinical Relevance of Time-to-positivity in BACTEC9240 Blood Culture System
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Park SH, Shim H, Yoon NS, Kim MN
- KMID: 1781627
- Korean J Lab Med.
- 2010 Jun;30(3):276-283.
- doi: 10.3343/kjlm.2010.30.3.276
BACKGROUND: Continuous monitoring systems have allowed determination of the time-to-positivity (TTP). We evaluated the clinical relevance of TTP in the BACTEC9240 system (Becton-Dickinson, USA). METHODS: A total of 2,354 vials... -
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- Performance of chromID Clostridium difficile Agar Compared with BBL C. difficile Selective Agar for Detection of C. difficile in Stool Specimens
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Han SB, Chang J, Shin SH, Park KG, Lee GD, Park YG, Park YJ
- KMID: 1791953
- Ann Lab Med.
- 2014 Sep;34(5):376-379.
- doi: 10.3343/alm.2014.34.5.376
We evaluated the performance of a new chromogenic medium for detection of Clostridium difficile, chromID C. difficile agar (CDIF; bioMerieux, France), by comparison with BBL C. difficile Selective Agar (CDSA;... -
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- Early Detection of Mycobacteria Using a Novel Hydrogel Culture Method
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Jang MH, Kim SY, Kim CK, Hwang SH, Park BK, Kim SS, Lee EY, Chang CL
- KMID: 1781356
- Ann Lab Med.
- 2014 Jan;34(1):26-30.
- doi: 10.3343/alm.2014.34.1.26
BACKGROUND: Early laboratory detection of Mycobacterium tuberculosis is crucial for controlling tuberculosis. We developed a hydrogel mycobacterial culture method that retains the advantages of both solid and liquid methods in... -
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- Acceptability of Sputum Specimens for Diagnosing Pulmonary Tuberculosis
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Lee YJ, Shin S, Roh EY, Yoon JH, Kim DK, Chung HS, Lee CH
- KMID: 2160601
- J Korean Med Sci.
- 2015 Jun;30(6):733-736.
- doi: 10.3346/jkms.2015.30.6.733
The evaluation of the quality of a sputum specimen prior to bacterial culture has been an accepted practice. However, optimal sputum criteria for pulmonary tuberculosis (TB) are not well established.... -
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- Comparative Evaluation of Several Gene Targets for Designing a Multiplex-PCR for an Early Diagnosis of Extrapulmonary Tuberculosis
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Raj A, Singh N, Gupta KB, Chaudhary , Yadav A, Chaudhary , Agarwal K, Varma-Basil M, Prasad R, Khuller GK, Mehta PK
- KMID: 2466356
- Yonsei Med J.
- 2016 Jan;57(1):88-96.
- doi: 10.3349/ymj.2016.57.1.88
PURPOSE: Diagnosis of extrapulmonary tuberculosis (EPTB) poses serious challenges. A careful selection of appropriate gene targets is essential for designing a multiplex-polymerase chain reaction (M-PCR) assay. MATERIALS AND METHODS: We compared... -
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- Evaluation of Dipicolinic Acid-Based Mueller Hinton Agar Biplate for Detection of IMP-1 and VIM-2 type Metallo-beta-Lactamase in Imipenem Non-susceptible Gram Negative Bacilli
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Shin KS, Son BR, Koo SH, Lee SH, Ahn JB, Park SH, Hwang SY
- KMID: 1096971
- Korean J Lab Med.
- 2009 Jun;29(3):204-211.
- doi: 10.3343/kjlm.2009.29.3.204
BACKGROUND: Since metallo-beta-lactamase (MBL)-producing isolates can hydrolyze carbapenem and also easily transfer the resistance genes to other bacteria, a rapid and accurate detection of MBL has become very important. We... -
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- Risk factors and etiology of surgical site infection after radical neck dissection in patients with head and neck cancer
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Park SY, Kim MS, Eom JS, Lee JS, Rho YS
- KMID: 2373785
- Korean J Intern Med.
- 2016 Jan;31(1):162-169.
- doi: 10.3904/kjim.2016.31.1.162
BACKGROUND/AIMS: Surgical site infection (SSI) is a major complication after radical neck dissection (RND) in patients with head and neck cancer (HNC). We investigated the incidence, risk factors, and etiology... -
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- Evaluation of PCR-Reverse Blot Hybridization Assay, REBA Sepsis-ID Test, for Simultaneous Identification of Bacterial Pathogens and mecA and van Genes from Blood Culture Bottles
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Park SD, Lee G, Wang HY, Park M, Kim S, Kim H, Kim J, Kim YK, Kim HY, Lee H, Uh Y, Kim JB
- KMID: 2129570
- Ann Lab Med.
- 2014 Nov;34(6):446-455.
- doi: 10.3343/alm.2014.34.6.446
BACKGROUND: The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia... -
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