Korean J Lab Med.  2010 Apr;30(2):133-137. 10.3343/kjlm.2010.30.2.133.

Development and Evaluation of the Quick Anaero-system-A New Disposable Anaerobic Culture System

Affiliations
  • 1Department of Microbiology, Chosun University Medical School, Gwangju, Korea.
  • 2Department of Ophthalmology, Chosun University Medical School, Gwangju, Korea.
  • 3Department of Laboratory Medicine, Chosun University Medical School, Gwangju, Korea. sjbjang@chosun.ac.kr

Abstract

BACKGROUND
We developed a new disposable anaerobic culture system, namely, the Quick anaero-system, for easy culturing of obligate anaerobes.
METHODS
Our system consists of 3 components: 1) new disposable anaerobic gas pack, 2) disposable culture-envelope and sealer, and 3) reusable stainless plate rack with mesh containing 10 g of palladium catalyst pellets. To evaluate the efficiency of our system, we used 12 anaerobic bacteria. We prepared 2 sets of ten-fold serial dilutions of the 12 anaerobes, and inoculated these samples on Luria-Bertani (LB) broth and LB blood agar plate (LB-BAP) (BD Diagnostic Systems, USA). Each set was incubated in the Quick anaero-system (DAS Tech, Korea) and BBL GasPak jar with BD GasPak EZ Anaerobe Container System (BD Diagnostic Systems) at 35-37degrees C for 48 hr. The minimal inoculum size showing visible growth of 12 anaerobes when incubated in both the systems was compared.
RESULTS
The minimal inoculum size showing visible growth for 2 out of the 12 anaerobes in the LB broth and 9 out of the 12 anaerobes on LB-BAP was lower for the Quick anaero-system than in the BD GasPak EZ Anaerobe Container System. The mean time (+/-SD) required to achieve absolute anaerobic conditions of the Quick anaero-system was 17 min and 56 sec (+/-3 min and 25 sec).
CONCLUSIONS
The Quick anaero-system is a simple and effective method of culturing obligate anaerobes, and its performance is superior to that of the BD GasPak EZ Anaerobe Container System.

Keyword

Anaerobic bacteria; Culture techniques; Culture media

MeSH Terms

Bacteria, Anaerobic/*growth &development
Bacteriological Techniques/instrumentation/methods
Culture Media/chemistry
Gases/chemistry
Palladium/chemistry
Reagent Kits, Diagnostic
Full Text Links
  • KJLM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr