J Korean Med Sci.  1999 Apr;14(2):182-186. 10.3346/jkms.1999.14.2.182.

Detection of Helicobacter DNA in bile from bile duct diseases

• Roe IH
• Kim JT
• Lee HS
• Lee JH

• KMID: 648078
• DOI: http://doi.org/10.3346/jkms.1999.14.2.182

Abstract

Several species of Helicobacter colonize the hepatobiliary tract of animals and cause hepatobiliary diseases. The aim of this study is to investigate Helicobacter found in the biliary tract diseases of humans. Thirty-two bile samples (15 from bile duct cancer, 6 from pancreatic head cancer, and 11 from intrahepatic duct stone) were obtained by percutaneous transhepatic biliary drainage. Polymerase chain reaction analysis using Helicobacter specific urease A gene and 16S rRNA primers, bile pH measurement, and Helicobacter culture were performed. Helicobacter DNA was detected in 37.5%, and 31.3% by PCR with ureA gene, and 16S rRNA, respectively. The bile pH was not related to the presence of Helicobacter. The cultures were not successful. In conclusion, Helicobacter can be detected in the bile of patients with bile duct diseases. The possibility of pathogenesis of biliary tract diseases in humans by these organisms will be further investigated.

Keyword

Helicobacter; Bile; Biliary tract diseases; Polymerase chain reaction

MeSH Terms

Adenocarcinoma/microbiology
Adult
Aged
Aged, 80 and over
Bile/microbiology*
Bile Duct Diseases/microbiology*
Bile Duct Neoplasms/microbiology
Cholelithiasis/microbiology
DNA Primers
DNA, Bacterial*
Helicobacter/isolation & purification*
Helicobacter/growth & development
Helicobacter/genetics
Human
Hydrogen-Ion Concentration
Middle Age
Pancreatic Neoplasms/microbiology
Polymerase Chain Reaction