Korean J Med.  2000 May;58(5):526-531.

Identification of Helicobacter in diseased human bile

• Kim SB
• Lee BS
• Park TJ
• Ko MH
• Park HJ
• Roe IH
• Shin JH
• Lee JH

Abstract

BACKGROUND
Several studies have been reported that the presence of Helicobacter DNA in human bile sample, although its pathological role is not clear. The purpose of this study was to evaluate the presence and identification of Helicobacter species in human bile samples obtained from patients with biliary tract diseases.
METHODS
58 bile samples (35 intrahepatic duct stones, 10 bile duct cancer, 13 pancreatic cancer) were obtained by percutaneous transhepatic biliary drainage (PTBD). DNA was isolated from bile sample. The primers were designed to amplify region of Helicobacter genus specific 16S rRNA. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) was developed to differenciate the presence of H. pylori, H. bilis, H. rappini and H. muridarum.
RESULTS
Forty-two of 58 (72.4%) bile samples obtained from patients with biliary tract disease showed positive PCR band for Helicobacter genus specific 16S rRNA. H. pylori was found in 83.3% of positive samples. Either H. bilis or H. rappini was in 16.7%. H. muridarum, however, was not detected.
CONCLUSION
Helicobacter genus was detected in human bile samples obtained from patients with biliary tract diseases using PCR method, and the major species was H. pylori. In addition, RFLP technique was used successfully to identify Helicobacter species.

Keyword

Bile; Helicobacter; Helicobacter pylori; Polymerase chain reaction; Restriction fragment length polymorphism

MeSH Terms

Bile Duct Neoplasms
Bile*
Biliary Tract Diseases
DNA
Drainage
Helicobacter pylori
Helicobacter*
Humans*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
DNA