J Korean Med Sci.  1996 Dec;11(6):495-500. 10.3346/jkms.1996.11.6.495.

Effects of mixed leukocyte reaction, hydrocortisone and cyclosporine on expression of leukocyte adhesion molecules by endothelial and mesangial cells

• Ihm CG
• Hong SP
• Park JK
• Lee TW
• Cho BS
• Yang MH
• Kim MJ

• KMID: 645013
• DOI: http://doi.org/10.3346/jkms.1996.11.6.495

Abstract

We investigated the effects of mixed leukocyte reaction (MLR), hydrocortisone (HC) and cyclosporine A (CsA) on the expression of leukocyte adhesion molecules on the mesangial (MC) and endothelial cells (EnC). Cell surface enzyme immunoassay showed that INFnu, IL-1beta, or TNF alpha stimulated expression of ICAM-1, or VCAM-1 on MC after 24 hours. Flow cytometric analysis demonstrated that MLR supernatant induced a marked increase in mean fluorescence of or % of cells highly expressing intercellular adhesion molecule(ICAM)-1 or vascular cell adhesion molecule (VCAM)-1 on both cells after 24 hours (p<0.001). HC treatment(300 ng/ml) during MLR effectively inhibited MLR-induced upregulation of ICAM-1 and VCAM-1 on both cells (p<0.005). When MLR supernatant with HC was added to adhesion molecule assay, there was no inhibitory effect of HC on VCAM-1. CsA treatment (500 ng/ml) during MLR caused a modest decrease in upregulation of VCAM-1 on EnC (p<0.05), but had no effects on ICAM-1 on both cells. CsA directly decreased expression of VCAM-1 on MC. In conclusion, alloreactive lymphocytes and monocytes upregulate the expression of VCAM-1 and ICAM-1 on target cells probably by the mediation of cytokines. HC effectively prevents MLR-induced upregulation of VCAM-1 and ICAM-1. CsA does not increase the expression of VCAM-1 and ICAM-1.

Keyword

Alloreactive cells; ICAM-1; VCAM-1; Hydrocortisone; Cyclosporine

MeSH Terms

Cells, Cultured
Cyclosporine/*pharmacology
Endothelium, Vascular/drug effects/*immunology
Glomerular Mesangium/drug effects/*immunology
Human
Hydrocortisone/*pharmacology
Intercellular Adhesion Molecule-1/*biosynthesis
Interleukin-1/pharmacology
Leukocytes/drug effects/*metabolism
Lymphocyte Culture Test, Mixed
Monocytes/drug effects/metabolism
Tumor Necrosis Factor/pharmacology
Vascular Cell Adhesion Molecule-1/*biosynthesis