Korean J Nephrol.  1998 May;17(3):376-382.

Mononuclear Cells Stimulate Leukocyte Adhesion Molecule Expression and Fibronectin Secretion in Mesangial Cells

• Ihm CG
• Park JK
• Hong SP
• Lee TW
• Kim MJ
• Cho BS

Abstract

BACKGROUNDS: The mesangial extracellular matrix expansion appear to be important features of progressive glomerular diseases and the mononuclear cells may play a pivotal role in this process. METHOD: We evaluated the effects of supernatants of peripheral blood mononuclear cells (PBMCS) activated by concanavalin A on the expression of VCAM-1 and ICAM-1, the secretion of monocyte chemotactic protein-1(MCP-1), the thymidine incorporation, and the fibronectin production in MC by {3H}-thymidine incorporation, flow cytometry, or enzyme immunoassay.
RESULTS
1) Expression of VCAM-1 and ICAM-1 by MC was enhanced by 43% and 53%, respectively, after the addition of PBMCS after 24 hours, as compared with media(P<0.001). 2) Secretion of MCP-1 was not different between addtion of PBMCS and media alone. 3) PBMCS inhibited MC thymidine incorporation by 59%, as compared with media alone(P<0.001). 4) PBMCS enhanced the fibronectin secretion in MC by 36% after 96 hours, as compared with media alone(P<0.05).
CONCLUSION
PBMCS enhanced expression of VCAM-1 and ICAM-1 by MC which stimulated leukocyte infiltration into glomeruli. And PBMCS inhibited the thymidine incorporation and enhanced the fibronectin secretion by MC, leading to mesangial expansion and eventually glomerulosclerosis.

Keyword

Mononuclear cells; Mesangial cells; Leukocyte adhesion molecule; MCP-1; Fibronectin

MeSH Terms

Concanavalin A
Fibronectins*
Flow Cytometry
Glomerular Mesangium
Immunoenzyme Techniques
Intercellular Adhesion Molecule-1
Leukocytes*
Mesangial Cells*
Monocytes
Thymidine
Vascular Cell Adhesion Molecule-1
Concanavalin A
Fibronectins
Intercellular Adhesion Molecule-1
Thymidine
Vascular Cell Adhesion Molecule-1