Ann Clin Microbiol.  2024 Jun;27(2):143-147. 10.5145/ACM.2024.27.2.8.

Four genotypes of carbapenem-resistant Acinetobacter baumannii strains lacking OXA-23 production in Korea

• Kim YA
• Jeong SH
• Shin JH
• Shin KS
• Shin JH
• Kim YR
• Kim HS
• Uh Y
• Ryoo NH

• KMID: 2557874
• DOI: http://doi.org/10.5145/ACM.2024.27.2.8

Abstract

During nationwide Fantimicrobial surveillance (Korea Global Antimicrobial Resistance Surveillance System [Kor-GLASS]), the recent emergence of non-oxacillinase (OXA)-23 production by carbapenem-resistant Acinetobacter baumannii (CRAB) isolates was noted. In this study, we evaluated resistance mechanisms other than OXA-23 production to elucidate the shift in considerable CRAB clones. The presence of OXA carbapenemase genes, such as bla_OXA-23 , bla_OXA-24 , bla_OXA-58 , and bla_OXA-51-ISAba1, was determined by PCR. Other carbapenemase genes, such as bla_IMP , bla_VIM , bla_NDM , bla_KPC , bla_GES , and bla_OXA-48 , were determined using sequencing. Strains lacking carbapenemase genes were subjected to whole genome sequencing, and resistance genes were analyzed using ResFinder. Four CRAB strains were collected through a Kor-GLASS study in 2022, in which OXA-23 production was not identified. The carbapenemase genotypes of the four CRAB strains lacking bla_OXA-23 were bla_OXA-51 -ISAba1, bla_OXA-66/ACD25 , bla_OXA-182, and bla_NDM-1 . To the best of our knowledge, this is the first study to identify CRAB producing New Delhi metallo-β-lactamase (NDM)-1 in Korea. In conclusion, domestic CRAB resistance mechanisms may undergo subtle changes. Continuous observations are required to monitor the emergence of new clones.

Keyword

Carbapenem; Resistance; Acinetobacter baumannii; NDM-1; OXA-23

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